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Electrochemical aptasensor for NS1 detection: Towards a fast dengue biosensor.
Bachour Junior, Bassam; Batistuti, Marina Ribeiro; Pereira, Aline Sanches; de Sousa Russo, Elisa Maria; Mulato, Marcelo.
Afiliação
  • Bachour Junior B; Department of Physics, Faculty of Philosophy, Sciences and Letters at Ribeirão Preto, University of São Paulo, Brazil.
  • Batistuti MR; Department of Physics, Faculty of Philosophy, Sciences and Letters at Ribeirão Preto, University of São Paulo, Brazil. Electronic address: marbatistuti@usp.br.
  • Pereira AS; Department of Clinical Analyses, Toxicology and Food Science, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.
  • de Sousa Russo EM; Department of Clinical Analyses, Toxicology and Food Science, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.
  • Mulato M; Department of Physics, Faculty of Philosophy, Sciences and Letters at Ribeirão Preto, University of São Paulo, Brazil.
Talanta ; 233: 122527, 2021 Oct 01.
Article em En | MEDLINE | ID: mdl-34215030
Dengue is one of the most commonly neglected tropical diseases transmitted by Aedes aegypti infected with Dengue virus. This virus belongs to the gender Flavivirus and produces a non-structural protein 1 (NS1), which is an important biomarker found at high levels in blood in early disease stage. Therefore, this study focused on the development of an electrochemical biosensor for NS1 detection using DNA aptamers. Gold electrodes were co-immobilized with specific aptamers and 6-mercapto-1-hexanol (MCH) to obtain a self-assembled monolayer. The molar ratio between aptamers and MCH was optimized and the platform characterized by electrochemical impedance spectroscopy and atomic force microscopy. Bovine serum albumin was added in NS1 solution to stabilize it and block the surface to avoid non-specific interactions. The biosensor performance was tested with NS1 protein serotype 4 (in phosphate saline buffer and human serum) and with a solution of serotype 1 in human serum. The results showed a sensitivity of 2.9%, 2.7% and 1.7% per decade, respectively, and low limit of detection (0.05, 0.022 and 0.025 ng/mL). The platform was also tested with Envelope protein as negative control. Furthermore, the aptamer sensor was able to detect NS1 in clinical range and it is a promising candidate for a new class for miniaturized point-of-care device for different Dengue serotypes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Dengue / Aptâmeros de Nucleotídeos Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Talanta Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Dengue / Aptâmeros de Nucleotídeos Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Talanta Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda