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Altered IgA Response to Gut Bacteria Is Associated with Childhood Asthma in Peru.
Hsieh, Chyi-Song; Rengarajan, Sunaina; Kau, Andrew; Tarazona-Meza, Carla; Nicholson, Andrew; Checkley, William; Romero, Karina; Hansel, Nadia N.
Afiliação
  • Hsieh CS; Division of Rheumatology, John T. Milliken Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO; chsieh@wustl.edu nhansel1@jhmi.edu.
  • Rengarajan S; Division of Rheumatology, John T. Milliken Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO.
  • Kau A; Division of Allergy and Immunology, John T. Milliken Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO.
  • Tarazona-Meza C; Asociacion Benefica Prisma, PRISMA, Lima, Peru.
  • Nicholson A; Center for Global Non-Communicable Disease Research and Training, Johns Hopkins University School of Medicine, Baltimore, MD.
  • Checkley W; Program in Global Disease Epidemiology and Control, Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD; and.
  • Romero K; Center for Global Non-Communicable Disease Research and Training, Johns Hopkins University School of Medicine, Baltimore, MD.
  • Hansel NN; Program in Global Disease Epidemiology and Control, Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD; and.
J Immunol ; 207(2): 398-407, 2021 07 15.
Article em En | MEDLINE | ID: mdl-34193598
Alterations in gut microbiota in early life have been associated with the development of asthma; however, the role of gut bacteria or the IgA response to gut bacteria in school-aged children with asthma is unclear. To address this question, we profiled the microbial populations in fecal and nasal swab samples by 16S rRNA sequencing from 40 asthma and 40 control children aged 9-17 y from Peru. Clinical history and laboratory evaluation of asthma and allergy were obtained. Fecal samples were analyzed by flow cytometry and sorted into IgA+ and IgA- subsets for 16S rRNA sequencing. We found that the fecal or nasal microbial 16S rRNA diversity and frequency of IgA+ fecal bacteria did not differ between children with or without asthma. However, the α diversity of fecal IgA+ bacteria was decreased in asthma compared with control. Machine learning analysis of fecal bacterial IgA-enrichment data revealed loss of IgA binding to the Blautia, Ruminococcus, and Lachnospiraceae taxa in children with asthma compared with controls. In addition, this loss of IgA binding was associated with worse asthma control (Asthma Control Test) and increased odds of severe as opposed to mild to moderate asthma. Thus, despite little to no change in the microbiota, children with asthma exhibit an altered host IgA response to gut bacteria compared with control participants. Notably, the signature of altered IgA responses is loss of IgA binding, in particular to members of Clostridia spp., which is associated with greater severity of asthma.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Asma / Imunoglobulina A / Microbioma Gastrointestinal Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Adolescent / Adult / Child / Female / Humans / Male País/Região como assunto: America do sul / Peru Idioma: En Revista: J Immunol Ano de publicação: 2021 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Asma / Imunoglobulina A / Microbioma Gastrointestinal Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Adolescent / Adult / Child / Female / Humans / Male País/Região como assunto: America do sul / Peru Idioma: En Revista: J Immunol Ano de publicação: 2021 Tipo de documento: Article País de publicação: Estados Unidos