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High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells.
Girão-Silva, T; Fonseca-Alaniz, M H; Ribeiro-Silva, J C; Lee, J; Patil, N P; Dallan, L A; Baker, A B; Harmsen, M C; Krieger, J E; Miyakawa, A A.
Afiliação
  • Girão-Silva T; Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of Sao Paulo Medical School, Av. Dr. Eneas de Carvalho Aguiar, 44-Bloco II, 10 Andar, São Paulo, SP, 05403-000, Brazil.
  • Fonseca-Alaniz MH; Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of Sao Paulo Medical School, Av. Dr. Eneas de Carvalho Aguiar, 44-Bloco II, 10 Andar, São Paulo, SP, 05403-000, Brazil.
  • Ribeiro-Silva JC; Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of Sao Paulo Medical School, Av. Dr. Eneas de Carvalho Aguiar, 44-Bloco II, 10 Andar, São Paulo, SP, 05403-000, Brazil.
  • Lee J; Department of Biomedical Engineering, Institute for Cellular and Molecular Biology, Institute for Biomaterials, Drug Delivery and Regenerative Medicine, Institute for Computational Engineering and Sciences, University of Texas at Austin, Austin, TX, USA.
  • Patil NP; Department of Biomedical Engineering, Institute for Cellular and Molecular Biology, Institute for Biomaterials, Drug Delivery and Regenerative Medicine, Institute for Computational Engineering and Sciences, University of Texas at Austin, Austin, TX, USA.
  • Dallan LA; Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of Sao Paulo Medical School, Av. Dr. Eneas de Carvalho Aguiar, 44-Bloco II, 10 Andar, São Paulo, SP, 05403-000, Brazil.
  • Baker AB; Department of Biomedical Engineering, Institute for Cellular and Molecular Biology, Institute for Biomaterials, Drug Delivery and Regenerative Medicine, Institute for Computational Engineering and Sciences, University of Texas at Austin, Austin, TX, USA.
  • Harmsen MC; Laboratory for Cardiovascular Regenerative Medicine Research Group (CAVAREM), Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
  • Krieger JE; Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of Sao Paulo Medical School, Av. Dr. Eneas de Carvalho Aguiar, 44-Bloco II, 10 Andar, São Paulo, SP, 05403-000, Brazil. j.krieger@hc.fm.usp.br.
  • Miyakawa AA; Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of Sao Paulo Medical School, Av. Dr. Eneas de Carvalho Aguiar, 44-Bloco II, 10 Andar, São Paulo, SP, 05403-000, Brazil. ayumi.miyakawa@incor.usp.br.
Sci Rep ; 11(1): 13493, 2021 06 29.
Article em En | MEDLINE | ID: mdl-34188159
The rate of the remodeling of the arterialized saphenous vein conduit limits the outcomes of coronary artery bypass graft surgery (CABG), which may be influenced by endothelial dysfunction. We tested the hypothesis that high stretch (HS) induces human saphenous vein endothelial cell (hSVEC) dysfunction and examined candidate underlying mechanisms. Our results showed that in vitro HS reduces NO bioavailability, increases inflammatory adhesion molecule expression (E-selectin and VCAM1) and THP-1 cell adhesion. HS decreases F-actin in hSVECs, but not in human arterial endothelial cells, and is accompanied by G-actin and cofilin's nuclear shuttling and increased reactive oxidative species (ROS). Pre-treatment with the broad-acting antioxidant N-acetylcysteine (NAC) supported this observation and diminished stretch-induced actin remodeling and inflammatory adhesive molecule expression. Altogether, we provide evidence that increased oxidative stress and actin cytoskeleton remodeling play a role in HS-induced saphenous vein endothelial cell dysfunction, which may contribute to predisposing saphenous vein graft to failure.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Veia Safena / Estresse Mecânico / Actinas / Estresse Oxidativo / Células Endoteliais Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Veia Safena / Estresse Mecânico / Actinas / Estresse Oxidativo / Células Endoteliais Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido