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Transient Expression in Cytoplasm and Apoplast of Rotavirus VP6 Protein Fused to Anti-DEC205 Antibody in Nicotiana benthamiana and Nicotiana sylvestris.
Castillo-Esparza, J Francisco; Gómez-Lim, Miguel A.
Afiliação
  • Castillo-Esparza JF; Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN, 36824, Irapuato, Guanajuato, Mexico. jose.castillo@cinvestav.mx.
  • Gómez-Lim MA; Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN, 36824, Irapuato, Guanajuato, Mexico. miguel.gomez@cinvestav.mx.
Mol Biotechnol ; 63(10): 973-982, 2021 Oct.
Article em En | MEDLINE | ID: mdl-34146324
Rotavirus is the most common cause of severe diarrhea in infants and children worldwide and is responsible for about 215,000 deaths annually. Over 85% of these deaths originate in low-income/developing countries in Asia and Africa. Therefore, it is necessary to explore the development of vaccines that avoid the use of "living" viruses and furthermore, vaccines that have viral antigens capable of generating powerful heterotypic responses. Our strategy is based on the expression of the fusion of the anti-DEC205 single-chain variable fragment (scFv) coupled by an OLLAS tag to a viral protein (VP6) of Rotavirus in Nicotiana plants. It was possible to express transiently in N. benthamiana and N. sylvestris a recombinant protein consisting of the single chain variable fragment linked by an OLLAS tag to the VP6 protein. The presence of the recombinant protein, which had a molecular weight of approximately 75 kDa, was confirmed by immunodetection, in both plant species and in both cellular compartments (cytoplasm and apoplast) where it was expressed. In addition, the recombinant protein was modeled, and it was observed that some epitopes of interest are exposed on the surface, which could favor their immunogenic response.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nicotiana / Rotavirus / Proteínas do Capsídeo / Anticorpos de Cadeia Única / Antígenos Virais Tipo de estudo: Prognostic_studies Idioma: En Revista: Mol Biotechnol Assunto da revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nicotiana / Rotavirus / Proteínas do Capsídeo / Anticorpos de Cadeia Única / Antígenos Virais Tipo de estudo: Prognostic_studies Idioma: En Revista: Mol Biotechnol Assunto da revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Suíça