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Rational selection of hidden epitopes for a molecularly imprinted electrochemical sensor in the recognition of heat-denatured dengue NS1 protein.
Siqueira Silva, Matheus; Moreira Tavares, Ana Patricia; Leomil Coelho, Luiz Felipe; Morganti Ferreira Dias, Lígia Ely; Chura-Chambi, Rosa Maria; Guimarães da Fonseca, Flávio; Ferreira Sales, Maria Goreti; Costa Figueiredo, Eduardo.
Afiliação
  • Siqueira Silva M; Laboratory of Toxicant and Drug Analyses, Federal University of Alfenas, 37130-001, Alfenas, MG, Brazil.
  • Moreira Tavares AP; BioMark@ISEP, School of Engineering of the Polytechnic School of Porto, 4200-072, Paranhos, Portugal; BioMark@UC, Department of Chemical Engineering, Faculty of Sciences and Technology of the University of Coimbra, 3030-790, Coimbra, Portugal.
  • Leomil Coelho LF; Laboratory of Vaccines, Department of Microbiology and Immunology, Institute of Biomedical Sciences, Federal University of Alfenas, 37130-001, Alfenas, MG, Brazil.
  • Morganti Ferreira Dias LE; Center of Biotechnology, Institute of Energetic and Nuclear Research, IPEN-CNEN/SP, 05508-000, São Paulo, SP, Brazil.
  • Chura-Chambi RM; Center of Biotechnology, Institute of Energetic and Nuclear Research, IPEN-CNEN/SP, 05508-000, São Paulo, SP, Brazil.
  • Guimarães da Fonseca F; Institute of Biological Sciences, Federal University of Minas Gerais, 31270-901, Belo Horizonte, MG, Brazil.
  • Ferreira Sales MG; BioMark@ISEP, School of Engineering of the Polytechnic School of Porto, 4200-072, Paranhos, Portugal; BioMark@UC, Department of Chemical Engineering, Faculty of Sciences and Technology of the University of Coimbra, 3030-790, Coimbra, Portugal. Electronic address: goreti.sales@eq.uc.pt.
  • Costa Figueiredo E; Laboratory of Toxicant and Drug Analyses, Federal University of Alfenas, 37130-001, Alfenas, MG, Brazil. Electronic address: eduardo.figueiredo@unifal-mg.edu.br.
Biosens Bioelectron ; 191: 113419, 2021 Nov 01.
Article em En | MEDLINE | ID: mdl-34144470
Rational selection of predicted peptides to be employed as templates in molecular imprinting was carried out for the heat-denatured non-structural protein 1 (NS1) of dengue virus (DENV). Conservation analysis among 301 sequences of Brazilian isolates of DENV and zika virus (ZIKV) NS1 was carried out by UniProtKB, and peptide selection was based on in silico data of the conservational, structural and immunogenic properties of the sequences. The selected peptide (from dengue 1 NS1) was synthesized and employed as a template in the electropolymerization of polyaminophenol-imprinted films on the surface of carbon screen-printed electrodes. Heat denaturation of the protein was carried out prior to analysis, in order to expose its internal hidden epitopes. After removal of the template, the molecularly imprinted cavities were able to rebind to the whole denatured protein as determined by electrochemical impedance spectroscopy. This label-free sensor was efficient to distinguish the NS1 of DENV from the NS1 of ZIKV. Additionally, the sensor was also selective for dengue NS1, in comparison with human serum immunoglobulin G and human serum albumin. Additionally, the device was able to detect the DENV NS1 at concentrations from 50 to 200 µg L-1 (RSD below 5.04%, r = 0.9678) in diluted human serum samples. The calculated LOD and LOQ were, respectively, 29.3 and 88.7 µg L-1 and each sensor could be used for six sequential cycles with the same performance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Dengue / Vírus da Dengue / Zika virus / Infecção por Zika virus Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Biosens Bioelectron Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Dengue / Vírus da Dengue / Zika virus / Infecção por Zika virus Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Biosens Bioelectron Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido