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Bioelectric, tissue, and molecular characteristics of the gastric mucosa at different times of ischemia.
Eduardo, Peña-Mercado; Mario, Garcia-Lorenzana; Carlos César, Patiño-Morales; Mayra, Montecillo-Aguado; Sara, Huerta-Yepez; E, Beltran Nohra.
Afiliação
  • Eduardo PM; Posgrado en Ciencias Naturales e Ingenieria, Unidad Cuajimalpa, Universidad Autonoma Metropolitana, CDMX 05340, Mexico.
  • Mario GL; Departamento de Biologia de la Reproduccion, Unidad Iztapalapa, Universidad Autonoma Metropolitana, CDMX 09340, Mexico.
  • Carlos César PM; Laboratorio de Investigacion en Biologia del Desarrollo y Teratogenesis Experimental, Hospital Infantil de Mexico, Federico Gomez, CDMX 06720, Mexico.
  • Mayra MA; Doctorado en Ciencias Biologicas, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, CDMX 04510, Mexico.
  • Sara HY; Unidad de Investigacion en Enfermedades Hematoncologicas, Hospital Infantil de Mexico, Federico Gomez, CDMX 06720, Mexico.
  • E BN; Departamento de Procesos y Tecnologia, Unidad Cuajimalpa, Universidad Autonoma Metropolitana, CDMX 05340, Mexico.
Exp Biol Med (Maywood) ; 246(18): 1968-1980, 2021 09.
Article em En | MEDLINE | ID: mdl-34130514
Gastrointestinal ischemia may be presented as a complication associated with late shock detection in patients in critical condition. Prolonged ischemia can cause mucosal integrity to lose its barrier function, triggering alterations that can induce organ dysfunction and lead to death. Electrical impedance spectroscopy has been proposed to identify early alteration in ischemia-induced gastric mucosa in this type of patients. This work analyzed changes in impedance parameters, and tissue and molecular alterations that allow us to identify the time of ischemia in which the gastric mucosa still maintains its barrier function. The animals were randomly distributed in four groups: Control, Ischemia 60, 90, and 120 min. Impedance parameters were measured and predictive values were determined to categorize the degree of injury using a receiver operating characteristic curve. Markers of inflammatory process and apoptosis (iNOS, TNFα, COX-2, and Caspase-3) were analyzed. The largest increase in impedance parameters occurred in the ischemia 90 and 120 min groups, with resistance at low frequencies (RL) and reactance at high frequencies (XH) being the most related to damage, allowing prediction of the occurrence of reversible and irreversible tissue damage. Histological analysis and apoptosis assay showed progressive mucosal deterioration with irreversible damage (p < 0.001) starting from 90 min of ischemia. Furthermore, a significant increase in the expression of iNOS, TNFα, and COX-2 was identified in addition to apoptosis in the gastric mucosa starting from 90 min of ischemia. Tissue damage generated by an ischemia time greater than 60 min induces loss of barrier function in the gastric mucosa.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão / Mucosa Gástrica / Isquemia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Exp Biol Med (Maywood) Assunto da revista: BIOLOGIA / FISIOLOGIA / MEDICINA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão / Mucosa Gástrica / Isquemia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Exp Biol Med (Maywood) Assunto da revista: BIOLOGIA / FISIOLOGIA / MEDICINA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido