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Effects of essential oils on native and recombinant acetylcholinesterases of Rhipicephalus microplus.
Santos, Everton Gomes Guimarães Dos; Bezerra, Wallyson André Dos Santos; Temeyer, Kevin B; León, Adalberto A Pérez de; Costa-Junior, Livio Martins; Soares, Alexandra Martins Dos Santos.
Afiliação
  • Santos EGGD; Laboratório de Bioquímica Vegetal, Universidade Federal do Maranhão - UFMA, São Luís, MA, Brasil.
  • Bezerra WADS; Laboratório de Bioquímica Vegetal, Universidade Federal do Maranhão - UFMA, São Luís, MA, Brasil.
  • Temeyer KB; USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, Kerrville, TX, United States of America.
  • León AAP; USDA-ARS San Joaquin Valley Agricultural Sciences Center, Parlier, CA, United States of America.
  • Costa-Junior LM; Laboratório de Controle de Parasitas, Universidade Federal do Maranhão - UFMA, São Luís, MA, Brasil.
  • Soares AMDS; Laboratório de Bioquímica Vegetal, Universidade Federal do Maranhão - UFMA, São Luís, MA, Brasil.
Rev Bras Parasitol Vet ; 30(2): e002221, 2021.
Article em En | MEDLINE | ID: mdl-34076049
This study reports the action of essential oils (EO) from five plants on the activity of native and recombinant acetylcholinesterases (AChE) from Rhipicephalus microplus. Enzyme activity of native susceptible AChE extract (S.AChE), native resistant AChE extract (R.AChE), and recombinant enzyme (rBmAChE1) was determined. An acetylcholinesterase inhibition test was used to verify the effect of the EO on enzyme activity. EO from Eucalyptus globulus, Citrus aurantifolia, Citrus aurantium var.dulcis inhibited the activity of S.AChE and R.AChE. Oils from the two Citrus species inhibited S.AChE and R.AChE in a similar way while showing greater inhibition on R.AChE. The oil from E. globulus inhibited native AChE, but no difference was observed between the S.AChE and R.AChE; however, 71% inhibition for the rBmAChE1 was recorded. Mentha piperita oil also inhibited S.AChE and R.AChE, but there was significant inhibition at the highest concentration tested. Cymbopogon winterianus oil did not inhibit AChE. Further studies are warranted with the oils from the two Citrus species that inhibited R.AChE because of the problem with R. microplus resistant to organophosphates, which target AChE. C. winterianus oil can be used against R. microplus populations that are resistant to organophosphates because its acaricidal properties act by mechanism(s) other than AChE inhibition.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óleos Voláteis / Inibidores da Colinesterase / Cymbopogon / Rhipicephalus / Acaricidas Limite: Animals Idioma: En Revista: Rev Bras Parasitol Vet Assunto da revista: MEDICINA VETERINARIA / PARASITOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óleos Voláteis / Inibidores da Colinesterase / Cymbopogon / Rhipicephalus / Acaricidas Limite: Animals Idioma: En Revista: Rev Bras Parasitol Vet Assunto da revista: MEDICINA VETERINARIA / PARASITOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil