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Detection of Antimicrobial Residues in Poultry Litter: Monitoring a Risk through a Selective and Sensitive HPLC-MS/MS Method.
Yévenes, Karina; Pokrant, Ekaterina; Trincado, Lina; Lapierre, Lisette; Galarce, Nicolás; Martín, Betty San; Maddaleno, Aldo; Hidalgo, Héctor; Cornejo, Javiera.
Afiliação
  • Yévenes K; Department of Preventive Animal Medicine, Faculty of Veterinary and Animal Sciences, University of Chile, Santiago 8820808, Chile.
  • Pokrant E; Programa de Doctorado en Ciencias Silvoagropecuarias y Veterinarias, Campus Sur Universidad de Chile, Santiago 8820808, Chile.
  • Trincado L; Department of Preventive Animal Medicine, Faculty of Veterinary and Animal Sciences, University of Chile, Santiago 8820808, Chile.
  • Lapierre L; Programa de Doctorado en Ciencias Silvoagropecuarias y Veterinarias, Campus Sur Universidad de Chile, Santiago 8820808, Chile.
  • Galarce N; Department of Preventive Animal Medicine, Faculty of Veterinary and Animal Sciences, University of Chile, Santiago 8820808, Chile.
  • Martín BS; Programa de Doctorado en Ciencias Silvoagropecuarias y Veterinarias, Campus Sur Universidad de Chile, Santiago 8820808, Chile.
  • Maddaleno A; Department of Preventive Animal Medicine, Faculty of Veterinary and Animal Sciences, University of Chile, Santiago 8820808, Chile.
  • Hidalgo H; Department of Preventive Animal Medicine, Faculty of Veterinary and Animal Sciences, University of Chile, Santiago 8820808, Chile.
  • Cornejo J; Laboratory of Veterinary Pharmacology (FARMAVET), Faculty of Veterinary and Animal Sciences, University of Chile, Santiago 8820808, Chile.
Animals (Basel) ; 11(5)2021 May 14.
Article em En | MEDLINE | ID: mdl-34069030
Tetracyclines, sulphonamides, and quinolones are families of antimicrobials (AMs) widely used in the poultry industry and can excrete up to 90% of AMs administrated, which accumulate in poultry litter. Worryingly, poultry litter is widely used as an agriculture fertilizer, contributing to the spread AMs residues in the environment. The aim of this research was to develop a method that could simultaneously identify and quantify three AMs families in poultry litter by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Samples of AMs free poultry litter were used to validate the method according to 657/2002/EC and VICH GL49. Results indicate that limit of detection (LOD) ranged from 8.95 to 20.86 µg kg-1, while limits of quantitation (LOQ) values were between 26.85 and 62.58 µg kg-1 of tetracycline, 4-epi-tetracycline, oxytetracycline, 4-epi-oxytetracycline, enrofloxacin, ciprofloxacin, flumequine, sulfachloropyridazine, and sulfadiazine. Recoveries obtained ranged from 93 to 108%. The analysis of field samples obtained from seven commercial poultry flocks confirmed the adequacy of the method since it detected means concentrations ranging from 20 to 10,364 µg kg-1. This provides us an accurate and reliable tool to monitor AMs residues in poultry litter and control its use as agricultural fertilizer.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Etiology_studies / Risk_factors_studies Idioma: En Revista: Animals (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Chile País de publicação: Suíça
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Etiology_studies / Risk_factors_studies Idioma: En Revista: Animals (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Chile País de publicação: Suíça