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Antimycobacterial activity of Achyrocline flaccida (Asteraceae) aqueous extract from Southern Brazil.
Machado, Vanessa Schopf; Verdi, Camila Marina; Rossi, Grazielle Guidolin; Souza, Larissa Ilha de; Teixeira, Cristiane Antunes; Machado, Marina Lopes; da Silveira, Tássia Limana; Sagrillo, Michele Rorato; Machado, Alencar Kolinski; Dornelles, Rafaela Castro; Campos, Marli Matiko; Rodrigues, Eliseu; Emanuelli, Tatiana; Somacal, Sabrina; Santos, Roberto Christ Vianna.
Afiliação
  • Machado VS; Laboratory of Oral Microbiology Research (LAPEMICRO), Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Verdi CM; Laboratory of Oral Microbiology Research (LAPEMICRO), Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Rossi GG; Laboratory of Mycobacteriology (LABIMYCO), Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Souza LI; Laboratory of Oral Microbiology Research (LAPEMICRO), Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Teixeira CA; Laboratory of Oral Microbiology Research (LAPEMICRO), Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Machado ML; Laboratory of Neurotoxicology and Experimental Neuroprotection (LaNe2Ex), Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • da Silveira TL; Laboratory of Neurotoxicology and Experimental Neuroprotection (LaNe2Ex), Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Sagrillo MR; Cell Culture Laboratory, Postgraduate Program in Nanoscience, Franciscan University, Santa Maria, RS, Brazil.
  • Machado AK; Cell Culture Laboratory, Postgraduate Program in Nanoscience, Franciscan University, Santa Maria, RS, Brazil.
  • Dornelles RC; Chemistry Laboratory, Federal University of Pampa (UNIPAMPA), Bagé, RS, Brazil.
  • Campos MM; Laboratory of Mycobacteriology (LABIMYCO), Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Rodrigues E; Department of Food Science, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Emanuelli T; Integrated Center for Laboratory Analysis Development (NIDAL), Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Somacal S; Integrated Center for Laboratory Analysis Development (NIDAL), Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Santos RCV; Laboratory of Oral Microbiology Research (LAPEMICRO), Federal University of Santa Maria, Santa Maria, RS, Brazil.
Nat Prod Res ; 36(11): 2897-2901, 2022 Jun.
Article em En | MEDLINE | ID: mdl-34039226
Achyrocline flaccida aqueous extract was obtained by macerating wildflowers. The phytochemical profile present in the A. flaccida aqueous extract was elucidated by HPLC-ESI-MS/MS. Toxicity was evaluated in vitro by comet assay in peripheral blood mononuclear cells (PBMCs) and in vivo using Caenorhabditis elegans as a model. The antioxidant activity was also evaluated, and antimycobacterial activity was assessed by the broth microdilution method. The compounds present in the aqueous extract mainly belonged to the flavonoid class (89%). The concentrations that showed protective effects in C. elegans against oxidative stress and antimycobacterial activity had no toxic effects. The antimycobacterial activity test demonstrated that the concentration of 1,560 µg mL-1 inhibited the growth and eradication of the mycobacterial tested strains. Based on our findings, the A. flaccida aqueous extract presents a viable potential in developing new phytotherapeutic drugs against mycobacteria of clinical relevance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Asteraceae / Achyrocline Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Nat Prod Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Asteraceae / Achyrocline Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Nat Prod Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido