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Effects of Porcine Immature Oocyte Vitrification on Actin Microfilament Distribution and Chromatin Integrity During Early Embryo Development in vitro.
López, Alma; Ducolomb, Yvonne; Casas, Eduardo; Retana-Márquez, Socorro; Betancourt, Miguel; Casillas, Fahiel.
Afiliação
  • López A; Biological and Health Sciences Program, Metropolitan Autonomous University-Iztapalapa, Mexico City, Mexico.
  • Ducolomb Y; Department of Health Sciences, Metropolitan Autonomous University-Iztapalapa, Mexico City, Mexico.
  • Casas E; Department of Health Sciences, Metropolitan Autonomous University-Iztapalapa, Mexico City, Mexico.
  • Retana-Márquez S; Department of Health Sciences, Metropolitan Autonomous University-Iztapalapa, Mexico City, Mexico.
  • Betancourt M; Department of Biology of Reproduction, Metropolitan Autonomous University-Iztapalapa, Mexico City, Mexico.
  • Casillas F; Department of Health Sciences, Metropolitan Autonomous University-Iztapalapa, Mexico City, Mexico.
Front Cell Dev Biol ; 9: 636765, 2021.
Article em En | MEDLINE | ID: mdl-33959606
Vitrification is mainly used to cryopreserve female gametes. This technique allows maintaining cell viability, functionality, and developmental potential at low temperatures into liquid nitrogen at -196°C. For this, the addition of cryoprotectant agents, which are substances that provide cell protection during cooling and warming, is required. However, they have been reported to be toxic, reducing oocyte viability, maturation, fertilization, and embryo development, possibly by altering cell cytoskeleton structure and chromatin. Previous studies have evaluated the effects of vitrification in the germinal vesicle, metaphase II oocytes, zygotes, and blastocysts, but the knowledge of its impact on their further embryo development is limited. Other studies have evaluated the role of actin microfilaments and chromatin, based on the fertilization and embryo development rates obtained, but not the direct evaluation of these structures in embryos produced from vitrified immature oocytes. Therefore, this study was designed to evaluate how the vitrification of porcine immature oocytes affects early embryo development by the evaluation of actin microfilament distribution and chromatin integrity. Results demonstrate that the damage generated by the vitrification of immature oocytes affects viability, maturation, and the distribution of actin microfilaments and chromatin integrity, observed in early embryos. Therefore, it is suggested that vitrification could affect oocyte repair mechanisms in those structures, being one of the mechanisms that explain the low embryo development rates after vitrification.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Cell Dev Biol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Cell Dev Biol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Suíça