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Hormonal and neural responses to restraint stress in an animal model of perimenopause in female rats.
Arikawe, Adesina Paul; Rorato, Rodrigo César; Gomes, Nathali; Elias, Lucila Leico; Anselmo-Franci, Janete.
Afiliação
  • Arikawe AP; Laboratory of Neuroendocrinology, Department of Basic and Oral Biology Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
  • Rorato RC; Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria.
  • Gomes N; Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
  • Elias LL; Departmento de Biofísica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
  • Anselmo-Franci J; Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
J Neuroendocrinol ; 33(5): e12976, 2021 Apr 26.
Article em En | MEDLINE | ID: mdl-33900672
The present study investigated the hormonal and neural responses to stress in a perimenopause animal model induced by 4-vinylcyclohexene diepoxide (VCD), which induces progressive follicular depletion in rodents, allowing studies on the transition to ovarian failure. Female rats, aged 28 days old, were s.c. injected for 15 consecutive days with corn oil or VCD. At 85 ± 5 days after the onset of treatment, the jugular vein was cannulated in the afternoon of metoestrus and in next morning (dioestrus) at 10.00 am, rats were subjected to 30 minutes of restraint stress. Blood samples were withdrawn before (-5 minutes), during (2, 5, 15 and 30 minutes) and after (45, 60 and 90 minutes) stress and plasma prolactin, progesterone and corticosterone levels were measured. Animals were perfused, brains processed for c-Fos/tyrosine hydroxylase (TH) in the locus coeruleus (LC) and c-Fos/corticotrophin-releasing factor (CRF) in the paraventricular nucleus (PVN). In unstressed rats the density of ß-endorphin fibres was assessed in LC and PVN. In VCD-treated rats, stress-induced prolactin peak was higher, basal and peak progesterone levels were lower, and both levels of corticosterone were similar to controls. However, the recovery period was longer for both adrenal hormones. In VCD-treated rats the number of c-Fos/TH and c-Fos/CRF-immunoreactive neurones was higher whereas the density of ß-endorphin fibres was lower in LC and PVN. We surmise that the hyperactivity of the LC and PVN neurones in VCD-treated rats may be a result of the lower progesterone levels that resulted in the decrease of ß-endorphin content in both nuclei, thus impairing the negative-feedback mechanism in the recovery period.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: J Neuroendocrinol Assunto da revista: ENDOCRINOLOGIA / NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: J Neuroendocrinol Assunto da revista: ENDOCRINOLOGIA / NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos