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Microglial synaptic pruning on axon initial segment spines of dentate granule cells: Sexually dimorphic effects of early-life stress and consequences for adult fear response.
Zetter, Mario A; Hernández, Vito S; Roque, Angélica; Hernández-Pérez, Oscar R; Gómora, María J; Ruiz-Velasco, Silvia; Eiden, Lee E; Zhang, Limei.
Afiliação
  • Zetter MA; Department of Physiology, School of Medicine, National Autonomous University of Mexico, Mexico City, Mexico.
  • Hernández VS; Department of Physiology, School of Medicine, National Autonomous University of Mexico, Mexico City, Mexico.
  • Roque A; Department of Physiology, School of Medicine, National Autonomous University of Mexico, Mexico City, Mexico.
  • Hernández-Pérez OR; Department of Physiology, School of Medicine, National Autonomous University of Mexico, Mexico City, Mexico.
  • Gómora MJ; Department of Embryology, School of Medicine, National Autonomous University of Mexico, Mexico City, Mexico.
  • Ruiz-Velasco S; Department of Probability and Statistics, Applied Mathematics and Systems Research Institute, National Autonomous University of Mexico, Mexico City, Mexico.
  • Eiden LE; Section on Molecular Neuroscience, Intramural Research Program, National Institute of Mental Health, NIH, Bethesda, MD, USA.
  • Zhang L; Department of Physiology, School of Medicine, National Autonomous University of Mexico, Mexico City, Mexico.
J Neuroendocrinol ; 33(7): e12969, 2021 07.
Article em En | MEDLINE | ID: mdl-33890333
Axon initial segments (AIS) of dentate granule cells in the hippocampus exhibit prominent spines (AISS) during early development that are associated with microglial contacts. In the present study, we investigated whether developmental changes in AISS could be modified by early-life stress (ELS), specifically neonatal maternal separation (MS), through stress hormones and microglial activation and examined the potential behavioural consequences. We examined AISS at postnatal day (PND)5, 15 and 50, using Golgi-Cox staining and anatomical analysis. Neurone-microglial interaction was assessed using antibodies against ankyrin-G, PSD-95 and Iba1, for AIS, AISS and microglia visualisation, respectively, in normally reared and neonatal maternally separated male and female rats. We observed a higher density of AISS in ELS rats at both PND15 and PND50 compared to controls. Effects were more pronounced in females than males. AIS-associated microglia in ELS rats showed a hyper-ramified morphology and less co-localisation with PSD-95 compared to controls at PND15. ELS-associated alteration in microglial morphology and synaptic pruning was mimicked by treatment of acute hippocampal slices of normally reared rats with vasopressin. ELS rats exhibited increased freezing behaviour during auditory fear memory testing, which was more pronounced in female subjects and corresponded with increased Fos expression in dorsal and ventral dentate granule cells. Thus, microglial synaptic pruning in dentate AIS of hippocampus is influenced by ELS, with demonstrable sex bias regarding its anatomical characteristics and subsequent fear-induced defensive behaviours.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estresse Psicológico / Microglia / Giro Denteado / Medo / Plasticidade Neuronal Limite: Animals / Pregnancy Idioma: En Revista: J Neuroendocrinol Assunto da revista: ENDOCRINOLOGIA / NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estresse Psicológico / Microglia / Giro Denteado / Medo / Plasticidade Neuronal Limite: Animals / Pregnancy Idioma: En Revista: J Neuroendocrinol Assunto da revista: ENDOCRINOLOGIA / NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos