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Use of equivalent circuit analysis and Cole-Cole model in evaluation of bioreactor operating conditions for biomass monitoring by impedance spectroscopy.
Díaz Pacheco, Adrián; Delgado-Macuil, Raul Jacobo; Díaz-Pacheco, Ángel; Larralde-Corona, Claudia Patricia; Dinorín-Téllez-Girón, Jabel; López-Y-López, Victor Eric.
Afiliação
  • Díaz Pacheco A; Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla, km 1.5, 90700, Tepetitla de Lardizábal, Tlaxcala, Mexico.
  • Delgado-Macuil RJ; Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla, km 1.5, 90700, Tepetitla de Lardizábal, Tlaxcala, Mexico.
  • Díaz-Pacheco Á; Departamento de Ciencias, Instituto Nacional de Astrofísica, Óptica Y Electrónica, Luis Enrique Erro No.1, Santa María Tonantzintla, 72840, San Andrés Cholula, Puebla, Mexico.
  • Larralde-Corona CP; Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Blvd. del Maestro S/N Esq. Elías Piña. Col. Narciso Mendoza, 88710, Reynosa, Tamaulipas, Mexico.
  • Dinorín-Téllez-Girón J; Universidad Politécnica de Tlaxcala, A. Universidad Politécnica No.1 San Pedro Xalcaltzinco, 90180, Tepeyanco, Tlaxcala, Mexico.
  • López-Y-López VE; Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla, km 1.5, 90700, Tepetitla de Lardizábal, Tlaxcala, Mexico. vlopezyl@ipn.mx.
Bioprocess Biosyst Eng ; 44(9): 1923-1934, 2021 Sep.
Article em En | MEDLINE | ID: mdl-33884467
The most important parameter in bioprocesses is biomass, where not only the quantity produced in a culture but also the behavior that is presented are important concerns. It is clear that conditions of operation in a bioreactor affect biomass production, but how operation conditions affect the measurement of biomass on-line is of special interest. We studied the effect of bioreactor operating condition variations on model parameters using impedance spectroscopy for biomass monitoring. The model parameters analyzed were capacitance, resistance, alpha (α), conductivity delta (∆σ) and critical frequency (fc). These model parameters were obtained by fitting data from impedance measurements to an equivalent circuit model and Cole-Cole conductivity model. The effect of operating conditions on the medium with no cells was estimated by the percentage of change in each model parameter. The operating conditions with the most significant percentage of change were determined, by comparing to the percentage of change of the same model parameters obtained, during a fermentation of Bacillus thuringiensis as a cellular model. Equivalent circuit parameters were mainly affected by variation in pH, temperature and aeration, whereas Cole-Cole parameters were affected by variation in agitation, aeration, temperature and pH. Therefore, any variation in these operating conditions (within the test interval) during a fermentation may generate changes in monitoring parameters, which will not be a direct consequence of any change in the properties of the biomass.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomassa / Reatores Biológicos / Espectroscopia Dielétrica Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomassa / Reatores Biológicos / Espectroscopia Dielétrica Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha