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Agathisflavone Modifies Microglial Activation State and Myelination in Organotypic Cerebellar Slices Culture.
de Almeida, Monique Marylin Alves; Pieropan, Francesca; Footz, Tim; David, Jorge Mauricio; David, Juceni Pereira; da Silva, Victor Diogenes Amaral; Dos Santos Souza, Cleide; Voronova, Anastassia; Butt, Arthur Morgan; Costa, Silvia Lima.
Afiliação
  • de Almeida MMA; Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, Bahia, Brazil.
  • Pieropan F; School of Pharmacy and Biomedical Science, University of Portsmouth, Portsmouth, UK.
  • Footz T; Department of Medical Genetics, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada.
  • David JM; School of Pharmacy and Biomedical Science, University of Portsmouth, Portsmouth, UK.
  • David JP; Department of Medical Genetics, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada.
  • da Silva VDA; Department of General and Inorganic Chemistry, Institute of Chemistry, Federal University of Bahia, Bahia, Brazil.
  • Dos Santos Souza C; Department of Medication, Faculty of Pharmacy, Federal University of Bahia, Bahia, Brazil.
  • Voronova A; Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, Bahia, Brazil.
  • Butt AM; Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, Bahia, Brazil.
  • Costa SL; Sheffield Institute for Translational Neuroscience, University of Sheffield, Sheffield, UK.
J Neuroimmune Pharmacol ; 17(1-2): 206-217, 2022 06.
Article em En | MEDLINE | ID: mdl-33881709
Oligodendrocytes produce the myelin that is critical for rapid neuronal transmission in the central nervous system (CNS). Disruption of myelin has devastating effects on CNS function, as in the demyelinating disease multiple sclerosis (MS). Microglia are the endogenous immune cells of the CNS and play a central role in demyelination and repair. There is a need for new potential therapies that regulate myelination and microglia to promote repair. Agathisflavone (FAB) is a non-toxic flavonoid that is known for its anti-inflammatory and neuroprotective properties. Here, we examined the effects of FAB (5-50 µM) on myelination and microglia in organotypic cerebellar slices prepared from P10-P12 Sox10-EGFP and Plp1-DsRed transgenic mice. Immunofluorescence labeling for myelin basic protein (MBP) and neurofilament (NF) demonstrates that FAB significantly increased the proportion of MBP + /NF + axons but did not affect the overall number of oligodendroglia or axons, or the expression of oligodendroglial proteins CNPase and MBP. FAB is known to be a phytoestrogen, but blockade of α- or ß- estrogen receptors (ER) indicated the myelination promoting effects of FAB were not mediated by ER. Examination of microglial responses by Iba1 immunohistochemistry demonstrated that FAB markedly altered microglial morphology, characterized by smaller somata and reduced branching of their processes, consistent with a decreased state of activation, and increased Iba1 protein expression. The results provide evidence that FAB increases the extent of axonal coverage by MBP immunopositive oligodendroglial processes and has a modulatory effect upon microglial cells, which are important therapeutic strategies in multiple neuropathologies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Revista: J Neuroimmune Pharmacol Assunto da revista: ALERGIA E IMUNOLOGIA / FARMACOLOGIA / NEUROLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Limite: Animals Idioma: En Revista: J Neuroimmune Pharmacol Assunto da revista: ALERGIA E IMUNOLOGIA / FARMACOLOGIA / NEUROLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos