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Solid-state culture of Azospirillum brasilense: a reliable technology for biofertilizer production from laboratory to pilot scale.
Martínez-Ramírez, C; Esquivel-Cote, R; Ferrera-Cerrato, R; Martínez-Ruiz, J A; Rodríguez-Serrano, G; Saucedo-Castañeda, G.
Afiliação
  • Martínez-Ramírez C; Department of Biotechnology, Iztapalapa Campus, Metropolitan Autonomous University, 09340, Mexico City, Mexico.
  • Esquivel-Cote R; College of Postgraduates, Microbiology Area, Montecillo, Z.C. 56230, Mexico, State of Mexico, Mexico.
  • Ferrera-Cerrato R; College of Postgraduates, Microbiology Area, Montecillo, Z.C. 56230, Mexico, State of Mexico, Mexico.
  • Martínez-Ruiz JA; Department of Biotechnology, Iztapalapa Campus, Metropolitan Autonomous University, 09340, Mexico City, Mexico.
  • Rodríguez-Serrano G; Department of Biotechnology, Iztapalapa Campus, Metropolitan Autonomous University, 09340, Mexico City, Mexico.
  • Saucedo-Castañeda G; Department of Biotechnology, Iztapalapa Campus, Metropolitan Autonomous University, 09340, Mexico City, Mexico. saucedo@xanum.uam.mx.
Bioprocess Biosyst Eng ; 44(7): 1525-1538, 2021 Jul.
Article em En | MEDLINE | ID: mdl-33860372
A biofertilizer of Azospirillum brasilense was produced in solid-state culture (SSC) from laboratory to pilot scale. Similar operation conditions (continuous aeration and mild intermittent mixing) and two dimensionless numbers with similar L/D ratio and a similar working volume were applied to reach a scale-up factor of 75. An innovative bioreactor with rotating helical ribbons (15 kg wet matter) was used at pilot scale. A mathematical model was proposed and validated to evaluate the respirometry trends at laboratory and pilot scale exhibiting similar behavior. The cell viability was (1.3 ± 0.4) × 109 and (1.3 ± 0.3) × 109 colony-forming units per gram of initial dry mass at laboratory and pilot scale, at 36 and 43 h, respectively. A. brasilense maintains its viability twelve months of storage at 4 and 30 °C. This is the first report of A. brasilense being cultivated in SSC under controlled conditions. SSC processes involving unicellular microorganisms with tolerance to agitation are a promising technology to produce biofertilizers.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biotecnologia / Microbiologia Industrial / Azospirillum brasilense / Reatores Biológicos / Glicerol Tipo de estudo: Prognostic_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biotecnologia / Microbiologia Industrial / Azospirillum brasilense / Reatores Biológicos / Glicerol Tipo de estudo: Prognostic_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha