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Comparison of Two Approaches for the Metataxonomic Analysis of the Human Milk Microbiome.
Ruiz, Lorena; Alba, Claudio; García-Carral, Cristina; Jiménez, Esther A; Lackey, Kimberly A; McGuire, Michelle K; Meehan, Courtney L; Foster, James; Sellen, Daniel W; Kamau-Mbuthia, Elizabeth W; Kamundia, Egidioh W; Mbugua, Samwel; Moore, Sophie E; Prentice, Andrew M; Gindola K, Debela; Otoo, Gloria E; Pareja, Rossina G; Bode, Lars; McGuire, Mark A; Williams, Janet E; Rodríguez, Juan M.
Afiliação
  • Ruiz L; Department of Nutrition and Food Science, Complutense University of Madrid, Madrid, Spain.
  • Alba C; Department of Nutrition and Food Science, Complutense University of Madrid, Madrid, Spain.
  • García-Carral C; Department of Nutrition and Food Science, Complutense University of Madrid, Madrid, Spain.
  • Jiménez EA; Department of Nutrition and Food Science, Complutense University of Madrid, Madrid, Spain.
  • Lackey KA; Margaret Ritchie School of Family and Consumer Sciences, University of Idaho, Moscow, ID, United States.
  • McGuire MK; Margaret Ritchie School of Family and Consumer Sciences, University of Idaho, Moscow, ID, United States.
  • Meehan CL; Department of Anthropology, Washington State University, Pullman, WA, United States.
  • Foster J; Margaret Ritchie School of Family and Consumer Sciences, University of Idaho, Moscow, ID, United States.
  • Sellen DW; Dalla Lana School of Public Health, University of Toronto, Toronto, ON, Canada.
  • Kamau-Mbuthia EW; Department of Human Nutrition, Egerton University, Nakuru, Kenya.
  • Kamundia EW; Department of Human Nutrition, Egerton University, Nakuru, Kenya.
  • Mbugua S; Department of Human Nutrition, Egerton University, Nakuru, Kenya.
  • Moore SE; Division of Women's Health, King's College London, London, United Kingdom.
  • Prentice AM; MRC Unit, Serekunda, Gambia.
  • Gindola K D; MRC International Nutrition Group, London School of Hygiene and Tropical Medicine, London, United Kingdom.
  • Otoo GE; Department of Anthropology, Hawassa University, Hawassa, Ethiopia.
  • Pareja RG; Department of Nutrition and Food Science, University of Ghana, Accra, Ghana.
  • Bode L; Instituto de Investigación Nutricional, Lima, Peru.
  • McGuire MA; Department of Pediatrics and Larsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence (MOMI CoRE), University of California, San Diego, La Jolla, CA, United States.
  • Williams JE; Department of Animal and Veterinary Science, University of Idaho, Moscow, ID, United States.
  • Rodríguez JM; Department of Animal and Veterinary Science, University of Idaho, Moscow, ID, United States.
Front Cell Infect Microbiol ; 11: 622550, 2021.
Article em En | MEDLINE | ID: mdl-33842385
Recent work has demonstrated the existence of large inter-individual and inter-population variability in the microbiota of human milk from healthy women living across variable geographical and socio-cultural settings. However, no studies have evaluated the impact that variable sequencing approaches targeting different 16S rRNA variable regions may have on the human milk microbiota profiling results. This hampers our ability to make meaningful comparisons across studies. In this context, the main purpose of the present study was to re-process and re-sequence the microbiome in a large set of human milk samples (n = 412) collected from healthy women living at diverse international sites (Spain, Sweden, Peru, United States, Ethiopia, Gambia, Ghana and Kenya), by targeting a different 16S rRNA variable region and reaching a larger sequencing depth. Despite some differences between the results obtained from both sequencing approaches were notable (especially regarding alpha and beta diversities and Proteobacteria representation), results indicate that both sequencing approaches revealed a relatively consistent microbiota configurations in the studied cohorts. Our data expand upon the milk microbiota results we previously reported from the INSPIRE cohort and provide, for the first time across globally diverse populations, evidence of the impact that different DNA processing and sequencing approaches have on the microbiota profiles obtained for human milk samples. Overall, our results corroborate some similarities regarding the microbial communities previously reported for the INSPIRE cohort, but some differences were also detected. Understanding the impact of different sequencing approaches on human milk microbiota profiles is essential to enable meaningful comparisons across studies. Clinical Trial Registration: www.clinicaltrials.gov, identifier NCT02670278.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microbiota / Leite Humano Tipo de estudo: Prognostic_studies Limite: Female / Humans País/Região como assunto: Africa / America do sul / Europa / Peru Idioma: En Revista: Front Cell Infect Microbiol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Espanha País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microbiota / Leite Humano Tipo de estudo: Prognostic_studies Limite: Female / Humans País/Região como assunto: Africa / America do sul / Europa / Peru Idioma: En Revista: Front Cell Infect Microbiol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Espanha País de publicação: Suíça