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Stress Response, Immunity, and Organ Mass in Toads (Rhinella diptycha) Living in Metal-Contaminated Areas.
Vasconcelos-Teixeira, Ronyelle; Titon, Stefanny C M; Titon, Braz; Pompêo, Marcelo L M; Gomes, Fernando R; Assis, Vania R.
Afiliação
  • Vasconcelos-Teixeira R; Laboratory of Behavior and Evolutionary Physiology, lab. 206, Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil. ronyellevasconcelos@gmail.com.
  • Titon SCM; Laboratory of Behavior and Evolutionary Physiology, lab. 206, Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Titon B; Laboratory of Disease & Drug-Associated Receptors Knowledge, Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Pompêo MLM; Laboratory of Ecophysiology and Evolutionary Physiology, Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Gomes FR; Laboratory of Limnology, Department of Ecology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Assis VR; Laboratory of Behavior and Evolutionary Physiology, lab. 206, Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
Biol Trace Elem Res ; 200(2): 800-811, 2022 Feb.
Article em En | MEDLINE | ID: mdl-33840055
Mining is one of the main activities that drive the economy of Brazil. Mining activity is associated with risk of contamination of environment and local fauna by metals. Amphibians have a life cycle that requires a transition between aquatic and terrestrial environments, increasing their vulnerability to metal contamination in the water and substrate. Metals are ubiquitous, with high bioaccumulative and biomagnifying potential, and may lead to immune and endocrine disruption. In this study, we analyzed two different components of the innate immune response, bacterial killing ability (BKA) and phytohemagglutinin edema (PHA), and two stress biomarkers, corticosterone plasma levels (CORT) and the neutrophil to lymphocyte ratio (N:L), of toads (Rhinella diptycha) living in places contaminated by metals. Blood samples were collected pre- and post-restraint (1h), followed by an immune challenge with PHA and tissue collection (liver, spleen, and kidneys). Toads liver metal bioaccumulation did not correlate with the immune response or stress biomarkers. Post-restraint, animals had increased CORT and reduced BKA, independently of the collection site, and these variables were not correlated with liver metal bioaccumulation. Interestingly, toads with the larger spleen (immune organ) showed increased N:L post-restraint and greater edema after the PHA challenge. Our results indicate that toads living in metal-contaminated environments responded to acute stressor, activating the hypothalamic-pituitary-interrenal axis and the immune response. Keep tracking the physiological variables of these animals and the presence of metals in the environment and tissues should provide valuable health status indicators for the population, which is vital for proposing amphibian conservation strategies in these areas.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bufonidae / Corticosterona Aspecto: Patient_preference Limite: Animals Idioma: En Revista: Biol Trace Elem Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bufonidae / Corticosterona Aspecto: Patient_preference Limite: Animals Idioma: En Revista: Biol Trace Elem Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos