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Molecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast.
de Sousa, Thatyane Pereira; Chaibub, Amanda Abdallah; Cortes, Marcio Vinicius de Carvalho Barros; Batista, Telma Fátima Coelho; Bezerra, Gustavo de Andrade; da Silva, Gisele Barata; de Filippi, Marta Cristina Corsi.
Afiliação
  • de Sousa TP; Agronomy School, Federal University of Goiás, Goiânia, GO, 74 690-900, Brazil.
  • Chaibub AA; Agronomy School, Federal University of Goiás, Goiânia, GO, 74 690-900, Brazil.
  • Cortes MVCB; Agricultural Microbiology Laboratory, Embrapa Rice and Beans, Santo Antônio de, Goiás, GO, 75375-000, Brazil.
  • Batista TFC; Plant Protection Laboratory, Institute of Agrarian Sciences, Federal Rural University of Amazonia., Campus Belém, Pará, Brazil.
  • Bezerra GA; Agronomy School, Federal University of Goiás, Goiânia, GO, 74 690-900, Brazil.
  • da Silva GB; Plant Protection Laboratory, Institute of Agrarian Sciences, Federal Rural University of Amazonia., Campus Belém, Pará, Brazil.
  • de Filippi MCC; Agricultural Microbiology Laboratory, Embrapa Rice and Beans, Santo Antônio de, Goiás, GO, 75375-000, Brazil. cristina.filippi@embrapa.com.
Arch Microbiol ; 203(6): 3257-3268, 2021 Aug.
Article em En | MEDLINE | ID: mdl-33837802
This study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated in two assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired-91.18% by Ufra.T09, volatile metabolites-all isolates equally reduced, non-volatile-68.33% by Ufra.T06, thermostability-99.77% by Ufra.T52 and co-cultivate-64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leaf blast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ascomicetos / Oryza / Hypocreales / Antibiose Tipo de estudo: Diagnostic_studies Idioma: En Revista: Arch Microbiol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ascomicetos / Oryza / Hypocreales / Antibiose Tipo de estudo: Diagnostic_studies Idioma: En Revista: Arch Microbiol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha