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Modulating photochemical reactions in Langmuir monolayers of Escherichia coli lipid extract with the binding mechanisms of eosin decyl ester and toluidine blue-O photosensitizers.
Moreira, Lucas G; Almeida, Alexandre M; Nield, Tyler; Camacho, Sabrina A; Aoki, Pedro H B.
Afiliação
  • Moreira LG; Saõ Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP, 19806-900, Brazil.
  • Almeida AM; Saõ Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP, 19806-900, Brazil.
  • Nield T; Saõ Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP, 19806-900, Brazil; Faculty of Engineering, University of Victoria, Victoria, BC V8P 5C2, Canada.
  • Camacho SA; Saõ Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP, 19806-900, Brazil; IFSC, São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP 13566-590, Brazil.
  • Aoki PHB; Saõ Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP, 19806-900, Brazil. Electronic address: pedro.aoki@unesp.br.
J Photochem Photobiol B ; 218: 112173, 2021 May.
Article em En | MEDLINE | ID: mdl-33799010
Photodynamic damage to the cell envelope can inactivate microorganisms and may be applied to combat super-resistance phenomenon, empowered by the indiscriminate use of antibiotics. Efficiency in microbial inactivation is dependent on the incorporation of photosensitizers (PS) into the bacterial membranes to trigger oxidation reactions under illumination. Herein, Langmuir monolayers of Escherichia coli lipid extract were built to determine the binding mechanisms and oxidation outcomes induced by eosin decyl ester (EosDEC) and toluidine blue-O (TBO) PSs. Surface-pressure isotherms of the E. coli monolayers were expanded upon EosDEC and TBO, suggesting incorporation of both PSs. Fourier-transform infrared spectroscopy (FTIR) of Langmuir-Schaefer (LS) films reveled that the EosDEC and TBO binding mechanisms are dominated by electrostatic interactions with the anionic polar groups, with limited penetration into the chains. Light-irradiation reduced the relative area of E. coli monolayer on TBO, indicating an increased loss of material to the subphase owing to the chain cleavage, generated by contact-dependent reactions with excited states of TBO. In contrast, the increased relative area of E. coli monolayers containing EosDEC suggests lipid hydroperoxidation, which is PS contact-independent. Even considering a small chain penetration, the saturated EosDEC may have partitioned towards saturated reach domains, avoiding direct contact with membrane unsaturations.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cloreto de Tolônio / Fármacos Fotossensibilizantes / Amarelo de Eosina-(YS) / Misturas Complexas / Escherichia coli / Lipídeos Idioma: En Revista: J Photochem Photobiol B Assunto da revista: BIOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cloreto de Tolônio / Fármacos Fotossensibilizantes / Amarelo de Eosina-(YS) / Misturas Complexas / Escherichia coli / Lipídeos Idioma: En Revista: J Photochem Photobiol B Assunto da revista: BIOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça