Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low cost.

Matsuda, Elaine Monteiro; de Campos, Ivana Barros; de Oliveira, Isabela Penteriche; Colpas, Daniela Rodrigues; Carmo, Andreia Moreira Dos Santos; Brígido, Luís Fernando de Macedo

Matsuda, Elaine Monteiro; de Campos, Ivana Barros; de Oliveira, Isabela Penteriche; Colpas, Daniela Rodrigues; Carmo, Andreia Moreira Dos Santos; Brígido, Luís Fernando de Macedo.

Afiliação

Matsuda EM; Infectious Diseases Outpatient Clinic, Santo André Health Secretary, Santo André, São Paulo, Brazil.
de Campos IB; Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil.
de Oliveira IP; Bioprocess and Biotecnology Engeneering, São Paulo State University, Botucatu, São Paulo, Brazil.
Colpas DR; Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil.
Carmo AMDS; Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil.
Brígido LFM; Virology Center, Adolfo Lutz Institute, São Paulo, São Paulo, Brazil.

J Med Virol ; 93(7): 4405-4410, 2021 07.

Article em En | MEDLINE | ID: mdl-33788270

RESUMO

One year into the coronavirus disease 2019 (COVID-19) pandemic, diagnostic strategies, although central for contact tracing and other preventive measures, are still limited. To meet the global demand, lower cost and faster antigen tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection are a convenient alternative to the gold standard reverse transcription-polymerase chain reaction (RT-PCR) assay. We tested laboratory-based RT-PCR RNA detection and two rapid antigen detection (RAD) tests, based on the immunochromatography test for nucleocapsid protein of SARS-CoV-2 (COVID-19 Ag ECO Test, ECO Diagnóstica, and Panbio COVID-19 Ag Rapid Test Abbott). Paired collection and testing were done in a small prospective open study in three clinical services in São Paulo, constituted of mostly symptomatic volunteers at collection (97%, 109/112) for a median of 4 days (interquartile range: 3-6), ranging from 1 to 30. Among the 108 paired RT-PCR/RAD tests, results were concordant in 96.4% (101/108). The test's performance was comparable, with an overall sensitivity of 87% and a specificity of 96%. These observations add to other data that suggest that antigen tests may provide reasonable sensitivity and specificity and deserve a role to improve testing strategies, especially in resource-limited settings.

Assuntos

Antígenos Virais/análise; Teste de Ácido Nucleico para COVID-19/métodos; Teste Sorológico para COVID-19/métodos; COVID-19/diagnóstico; RNA Viral/análise; Adolescente; Adulto; Idoso; Idoso de 80 Anos ou mais; Brasil; Criança; Pré-Escolar; Testes Diagnósticos de Rotina; Feminino; Humanos; Lactente; Masculino; Pessoa de Meia-Idade; Nasofaringe/virologia; Estudos Prospectivos; SARS-CoV-2/genética; SARS-CoV-2/imunologia; Sensibilidade e Especificidade; Adulto Jovem

Palavras-chave

COVID-19; SARS-CoV-2; diagnostic; gargle lavage; nasopharyngeal swab; rapid antigen detection

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Viral / Teste de Ácido Nucleico para COVID-19 / Teste Sorológico para COVID-19 / COVID-19 / Antígenos Virais Tipo de estudo: Diagnostic_studies / Evaluation_studies / Health_economic_evaluation / Observational_studies Limite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Female / Humans / Infant / Male País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Med Virol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

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