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In vivo tumor growth inhibition by Solanum tuberosum aspartic protease 3 (StAP3) treatment.
Ibañez, Irene L; Muñoz, Fernando F; Zoppi, Jorge; Abaurrea, Ricardo A; Scandogliero, Eduardo A; Durán, Hebe; Guevara, María Gabriela.
Afiliação
  • Ibañez IL; Institute of Nanoscience and Nanotechnology (INN), National Atomic Energy Commission (CNEA), National Scientific and Technical Research Council (CONICET), Constituyentes Node, Av. General Paz 1499, (B1650KNA) San Martín, Buenos Aires, Argentina.
  • Muñoz FF; Biological Research Institute, National Council of Scientific and Technique Research (IIB-CONICET), Funes 3250 7600, Mar del Plata, Argentina; National University of Mar del Plata, School of Science, 7600 Mar del Plata, Argentina.
  • Zoppi J; Hospital of Community. Laboratory of Pathology B7602CMB Mar del Plata, Argentina.
  • Abaurrea RA; Laboratory of Clinical and Bacteriological Analysis (BAS), 7600 Mar del Plata, Argentina.
  • Scandogliero EA; Laboratory of Clinical and Bacteriological Analysis (BAS), 7600 Mar del Plata, Argentina.
  • Durán H; Institute of Nanoscience and Nanotechnology (INN), National Atomic Energy Commission (CNEA), National Scientific and Technical Research Council (CONICET), Constituyentes Node, Av. General Paz 1499, (B1650KNA) San Martín, Buenos Aires, Argentina. Electronic address: hduran@cnea.gov.ar.
  • Guevara MG; Biological Research Institute, National Council of Scientific and Technique Research (IIB-CONICET), Funes 3250 7600, Mar del Plata, Argentina; National University of Mar del Plata, School of Science, 7600 Mar del Plata, Argentina. Electronic address: gguevara@mdp.edu.ar.
Bioorg Med Chem Lett ; 41: 127959, 2021 06 01.
Article em En | MEDLINE | ID: mdl-33766772
Solanum tuberosum aspartic Proteases (StAPs) show selective plasma membrane permeabilization, inducing cytotoxicity of cancer cells versus normal cells in vitro. Herein, we aimed to evaluate both StAP3 systemic toxicity and antitumoral activity against human melanoma in vivo. The toxicity of a single high dose of StAP3 (10 µg/g body weight, intraperitoneally) was assessed in a Balb/c mice model. Subcutaneous A375 human melanoma xenografts in athymic nude (nu/nu) mice were induced. Once tumors developed (mean larger dimension = 3.8 ± 0.09 mm), mice were StAP3-treated (6 µg/g body weight, subcutaneously under the tumor at a single dose). For both models, controls were treated with physiologic saline solution. StAP3-treated mice showed a significant inhibition of tumor growth (p < 0.05) compared with controls. No signs of toxicity were detected in StAP3-treated mice in both models. These results suggest the potential of these plant proteases as anticancer agents.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solanum tuberosum / Ácido Aspártico Proteases / Melanoma / Antineoplásicos Fitogênicos Limite: Animals / Humans Idioma: En Revista: Bioorg Med Chem Lett Assunto da revista: BIOQUIMICA / QUIMICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solanum tuberosum / Ácido Aspártico Proteases / Melanoma / Antineoplásicos Fitogênicos Limite: Animals / Humans Idioma: En Revista: Bioorg Med Chem Lett Assunto da revista: BIOQUIMICA / QUIMICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido