A biotechnological tool for glycoprotein desialylation based on immobilized neuraminidase from Clostridium perfringens.
Biochem Biophys Rep
; 26: 100940, 2021 Jul.
Article
em En
| MEDLINE
| ID: mdl-33732900
4 MU-NANA, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid; BCA, Bicinchonninic acid; CDAP-BF4, 1-Cyano-4-dimethylaminopyridinium tetrafluoroborate; ECA, Erythrina cristagalli lectin; ELISA-type assay, Enzyme-linked Immuno Sorbent assay; Enzymatic desialylation; FBS, Fetal bovine serum; Gal, Galactose; GalNAc, N-acetylgalactosamine; GlcNAc, N-acetylglucosamine; Glycomic analysis; Immobilization; Neu5Ac, N-Acetyl neuraminic acid; Neura-agarose, Neuraminidase immobilized onto agarose; Neuraminidase; OPD, ortho-Phenylendiamine; OSM, Ovine submaxilar mucin; PBS, Phosphate saline buffer; PE, Phycoerythrin; PNA, Arachis hipogaea lectin; SNA, Sambucus nigra lectin; Sialic acid; Sialyl-Tn antigen, Neu 5 Ac-2,6 GalNAc
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Biochem Biophys Rep
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Uruguai
País de publicação:
Holanda