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AIM2 inflammasome activation may mediate high mobility group box 1 release in murine allergic rhinitis.
Wang, Yan; Chen, Shan; Yang, Ping-Li; Chen, Jian-Jun; Kong, Wei-Jia; Wang, Yan-Jun.
Afiliação
  • Wang Y; Huazhong University of Science and Technology, Tongji Medical College, Union Hospital, Department of Otorhinolaryngology, Wuhan, China.
  • Chen S; Huazhong University of Science and Technology, Tongji Medical College, Union Hospital, Department of Otorhinolaryngology, Wuhan, China.
  • Yang PL; Shihezi University School of Medicine, The First Affiliated Hospital, Department of Otorhinolaryngology, Shihezi, China.
  • Chen JJ; Huazhong University of Science and Technology, Tongji Medical College, Union Hospital, Department of Otorhinolaryngology, Wuhan, China.
  • Kong WJ; Huazhong University of Science and Technology, Tongji Medical College, Union Hospital, Department of Otorhinolaryngology, Wuhan, China; Huazhong University of Science and Technology, Tongji Medical College, Union Hospital, Institute of Otorhinolaryngology, Wuhan, China. Electronic address: entwjkong
  • Wang YJ; Huazhong University of Science and Technology, Tongji Medical College, Union Hospital, Department of Otorhinolaryngology, Wuhan, China; Huazhong University of Science and Technology, Tongji Medical College, Union Hospital, Institute of Otorhinolaryngology, Wuhan, China. Electronic address: yjwang@hu
Braz J Otorhinolaryngol ; 88(6): 925-931, 2022.
Article em En | MEDLINE | ID: mdl-33707120
INTRODUCTION: High mobility group box 1 protein participates in the pathogenesis of allergic rhinitis. Activation of the inflammasome can mediate the release of high mobility group box 1. The role of the absent in melanoma 2 inflammasome in allergic rhinitis remains unclear. OBJECTIVE: This study aimed to investigate the function of absent in melanoma 2 inflammasome in murine allergic rhinitis and the interaction between high mobility group box 1 and the absent in melanoma 2 inflammasome. METHODS: A murine allergic rhinitis model was established using twenty Balb/c mice. Expression of the components of the absent in melanoma 2 inflammasome: absent in melanoma 2, apoptosis-associated speck-like protein containing a CARD (Asc), caspase-1 p20, and additional nod-like receptor family pyrin domain containing 3 (Nlrp3) were detected by western blotting during allergic rhinitis. Alterations of absent in melanoma 2, caspase-1, and high mobility group box 1 after ovalbumin challenge were demonstrated by immunohistochemistry. TdT-mediated dUTP Nick end labeling, TUNEL assay, and cleavage of caspase-3 and PARP-1 were used for the observation of pyroptosis. RESULTS: Eosinophilia and goblet cell infiltration were observed in the nasal mucosa of mice in the allergic rhinitis group. Absent in melanoma 2, Asc, and caspase-1 p20 increased after ovalbumin exposure while Nlrp3 did not. High mobility group box 1 was released in the nasal mucosa of allergic rhinitis mice. TUNEL-positive cells increased in the epithelium and laminae propria, whereas cleavage of caspase-3 and PARP-1 was not observed. CONCLUSIONS: The absent in melanoma 2 inflammasome was activated and pyroptosis may occur in the nasal mucosa after ovalbumin treatment. These may contribute to the translocation of high mobility group box 1 and the development of allergic rhinitis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteína HMGB1 / Proteínas de Ligação a DNA / Rinite Alérgica Limite: Animals Idioma: En Revista: Braz J Otorhinolaryngol Assunto da revista: OTORRINOLARINGOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteína HMGB1 / Proteínas de Ligação a DNA / Rinite Alérgica Limite: Animals Idioma: En Revista: Braz J Otorhinolaryngol Assunto da revista: OTORRINOLARINGOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Brasil