Your browser doesn't support javascript.
loading
Automated TTF-1 Immunohistochemistry Assay for the Differentiation of Lung Adenocarcinoma Versus Lung Squamous Cell Carcinoma.
Cintrón, Rosa Vélez; Martínez, Andrés J; Jusino, Jo Ann; Conte-Miller, María; Mendoza, Adalberto.
Afiliação
  • Cintrón RV; Southern Pathology Services Inc., Ponce, Puerto Rico. rvelez@southernpathology.com.
  • Martínez AJ; Pathology Division, Ponce Health Science University, Ponce, Puerto Rico. rvelez@southernpathology.com.
  • Jusino JA; School of Public Health, Ponce Health Science University, Ponce, Puerto Rico.
  • Conte-Miller M; Southern Pathology Services Inc., Ponce, Puerto Rico.
  • Mendoza A; Southern Pathology Services Inc., Ponce, Puerto Rico.
Methods Mol Biol ; 2279: 1-12, 2021.
Article em En | MEDLINE | ID: mdl-33683681
Due to therapeutic advances, the subclassification of non-small cell lung carcinomas (NSCLC) between the adenocarcinomas and squamous cell carcinomas subtypes is essential for the practice of personalized and targeted medicine. The clinical management for these two NSCLC subtypes is different due to their different molecular properties and histological origins. Immunohistochemistry (IHC) markers such is TTF-1 play a key role in the differentiation of lung adenocarcinomas and squamous cell carcinomas. However, immunohistochemistry is a complex process involving many critical steps and the reliability of results depends on the standardization of the assay as well as the appropriate interpretation. Different laboratories use different reagents and different IHC approaches for the detection of TTF-1 in lung cancer tumors. Here we describe an automated IHC protocol used in our laboratory for the detection of TTF-1 in formalin-fixed, paraffin-embedded (FFPE) tissue sections from lung tumors.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imuno-Histoquímica / Carcinoma de Células Escamosas / Automação Laboratorial / Adenocarcinoma de Pulmão / Neoplasias Pulmonares Tipo de estudo: Diagnostic_studies / Guideline Limite: Female / Humans / Male Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Porto Rico País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imuno-Histoquímica / Carcinoma de Células Escamosas / Automação Laboratorial / Adenocarcinoma de Pulmão / Neoplasias Pulmonares Tipo de estudo: Diagnostic_studies / Guideline Limite: Female / Humans / Male Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Porto Rico País de publicação: Estados Unidos