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Evaluation of Different Ionic Liquids as Additives in the Immobilization of Lipase CAL B by Sol-Gel Technique.
Ficanha, Aline Matuella Moreira; Oro, Carolina Elisa Demaman; Franceschi, Elton; Dallago, Rogério Marcos; Mignoni, Marcelo Luis.
Afiliação
  • Ficanha AMM; Department of Engineering, Centro de Ensino Riograndense, Marau, RS, CEP 99150000, Brazil. alinematuella@gmail.com.
  • Oro CED; Department of Food and Chemical Engineering, URI - Erechim, 1621, Sete de Setembro Av, Erechim, RS, 99709-910, Brazil. alinematuella@gmail.com.
  • Franceschi E; Department of Food and Chemical Engineering, URI - Erechim, 1621, Sete de Setembro Av, Erechim, RS, 99709-910, Brazil.
  • Dallago RM; Center for Studies on Colloidal Systems (NUESC)/Institute of Technology and Research (ITP), Postgraduate Programme in Industrial Biotechnology (PBI), Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju, SE, CEP 49032-490, Brazil.
  • Mignoni ML; Department of Food and Chemical Engineering, URI - Erechim, 1621, Sete de Setembro Av, Erechim, RS, 99709-910, Brazil.
Appl Biochem Biotechnol ; 193(7): 2162-2181, 2021 Jul.
Article em En | MEDLINE | ID: mdl-33683550
Sol-gel technique aiming enzymatic immobilization in situ with ionic liquids as additives is poorly studied. In this process, the addition of the enzyme is carried out in the synthesis of the support. The characteristics of ionic liquids, such as low vapor pressure, thermal stability, and non-flammability, make them strong candidates for use as immobilization additives. The objective of the present study was to immobilize the Candida antarctica B lipase by the sol-gel technique using ionic liquids as additives. The optimum points determined for ionic liquids 1-butyl-3-methylimidazolium chloride, 1-octyl-3-methylimidazolium bromide, and 1 hexadecyl-3-methylimimidazolium were 0.30, 0.27, and 0.22 g/mL of enzyme and 1.60, 1.52, and 1.52% of additive, respectively. The amount of enzyme and ionic liquids used in aerogel immobilization was the same as the optimized values in the xerogel immobilization process (for each ionic liquid). Ionic liquids proved to be good additives in the enzymatic immobilization process. Xerogel, regardless of the ionic liquid, presented a greater number of use cycles and better thermal stability compared to aerogel.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Basidiomycota / Proteínas Fúngicas / Líquidos Iônicos / Lipase Tipo de estudo: Evaluation_studies Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Basidiomycota / Proteínas Fúngicas / Líquidos Iônicos / Lipase Tipo de estudo: Evaluation_studies Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos