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Active Antioxidant Phenolics from Brazilian Red Propolis: An Optimization Study for Their Recovery and Identification by LC-ESI-QTOF-MS/MS.
Vieira de Morais, Daniel; Rosalen, Pedro Luiz; Ikegaki, Masaharu; de Souza Silva, Anna Paula; Massarioli, Adna Prado; de Alencar, Severino Matias.
Afiliação
  • Vieira de Morais D; Center for Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, SP 13416-000, Brazil.
  • Rosalen PL; Faculty of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, MG 37130-001, Brazil.
  • Ikegaki M; Faculty of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, MG 37130-001, Brazil.
  • de Souza Silva AP; Department of Agri-Food Industry, Food and Nutrition, 'Luiz de Queiroz' College of Agriculture, University of São Paulo, Piracicaba, SP 13416-000, Brazil.
  • Massarioli AP; Department of Agri-Food Industry, Food and Nutrition, 'Luiz de Queiroz' College of Agriculture, University of São Paulo, Piracicaba, SP 13416-000, Brazil.
  • de Alencar SM; Center for Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, SP 13416-000, Brazil.
Antioxidants (Basel) ; 10(2)2021 Feb 16.
Article em En | MEDLINE | ID: mdl-33669251
Brazilian red propolis (BRP) is a natural product widely known for its phenolic composition and strong antioxidant properties. In this study, we used the Box-Behnken Design (BBD) with Surface Response Methodology to optimize the extraction conditions for total phenolic content (TPC) and Trolox equivalent antioxidant capacity(TEAC) of bioactive phenolics from BRP. The extraction time, ethanol/water concentration and temperature, were tested. All variables had significant effects (p ≤ 0.05), with a desirability coefficient of 0.88. Under optimized conditions (90% ethanol at 80 °C for 30 min), the BRP extract showed a TPC of 129.00 ± 2.16 mg GAE/g and a TEAC of 3471.76 ± 53.86 µmol TE/g. Moreover, FRAP and ORAC assays revealed that the optimized BRP extract had 1472.86 ± 72.37 µmol Fe2+/g and 4339.61 ± 114.65 µmol TE/gof dry weight, respectively. Thirty-two phenolic compounds were tentatively identified by LC-QTOF-ESI-MS/MS, of which thirteen were found for the first time in BRP, including four flavones, one flavanol, two flavanones, two chalcones, and four isoflavonoids. Thus, our results highlight the importance of BRP as a source of a wide variety of phenolic compounds with significant antioxidant properties.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Antioxidants (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Antioxidants (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça