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Longitudinal changes in gray and white matter microstructure during epileptogenesis in pilocarpine-induced epileptic rats.
Luna-Munguia, Hiram; Marquez-Bravo, Luis; Concha, Luis.
Afiliação
  • Luna-Munguia H; Departamento de Neurobiologia Conductual y Cognitiva, Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico, Campus UNAM-Juriquilla, 76230, Queretaro, Mexico.
  • Marquez-Bravo L; Departamento de Neurobiologia Conductual y Cognitiva, Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico, Campus UNAM-Juriquilla, 76230, Queretaro, Mexico.
  • Concha L; Departamento de Neurobiologia Conductual y Cognitiva, Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico, Campus UNAM-Juriquilla, 76230, Queretaro, Mexico. Electronic address: lconcha@unam.mx.
Seizure ; 90: 130-140, 2021 Aug.
Article em En | MEDLINE | ID: mdl-33627232
PURPOSE: Temporal lobe epilepsy is associated with tissue abnormalities of several gray and white matter structures that are reproduced in animal models. Few longitudinal studies have focused on the identification of structural differences during epileptogenesis. The diffusion tensor model is a useful tool for evaluating cell death, gliosis, and axonal plasticity in epileptic subjects. This study aimed to evaluate temporal tissue changes after experimental status epilepticus in an animal model of chronic temporal lobe epilepsy. METHODS: Systemic pilocarpine-induced status epilepticus in adult Sprague-Dawley rats. Animals were scanned using diffusion tensor imaging (DTI) at three time points: prior to status epilepticus, and 24 and 64 days post-induction (early and late chronic, respectively). Fractional anisotropy, apparent diffusion coefficient, axial diffusivity (D║), and radial diffusivity (D┴) were evaluated in white (fimbria, cingulum, corpus callosum, and internal capsule) and gray (dorsal hippocampus, dentate gyrus, and CA3) matter regions for the three time points. Histological assessment of neurodegeneration in Klüver-Barrera preparations from the same animals was performed. RESULTS: Significantly reduced volume of dorsal hippocampus and fimbria of the epileptic animals was observed already at 24 days post-status epilepticus. Progressive changes of DTI parameters in both the white and gray matter structures of the experimental group were also observed. Stained sections confirmed such alterations. CONCLUSION: Our study revealed time-dependent diffusion changes in gray and white matter structures after pilocarpine-induced status epilepticus. The characterization of these alterations over time may be potential imaging markers for epileptogenesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Substância Branca Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Seizure Assunto da revista: NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Substância Branca Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Seizure Assunto da revista: NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido