Gene expression patterns associated with Leishmania panamensis infection in macrophages from BALB/c and C57BL/6 mice.

Restrepo, Carlos M; Llanes, Alejandro; Herrera, Lizzi; Ellis, Esteban; Lleonart, Ricardo; Fernández, Patricia L

Restrepo, Carlos M; Llanes, Alejandro; Herrera, Lizzi; Ellis, Esteban; Lleonart, Ricardo; Fernández, Patricia L.

Afiliação

Restrepo CM; Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, Panama, Republic of Panama.
Llanes A; Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, Panama, Republic of Panama.
Herrera L; Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, Panama, Republic of Panama.
Ellis E; Departamento de Biotecnología, Facultad de Ciencias de la Salud, Universidad Latina de Panamá, Panama City, Panama, Republic of Panama.
Lleonart R; Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, Panama, Republic of Panama.
Fernández PL; Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, Panama, Republic of Panama.

PLoS Negl Trop Dis ; 15(2): e0009225, 2021 02.

Article em En | MEDLINE | ID: mdl-33617537

RESUMO

Leishmania parasites can trigger different host immune responses that result in varying levels of disease severity. The C57BL/6 and BALB/c mouse strains are among the host models commonly used for characterizing the immunopathogenesis of Leishmania species and the possible antileishmanial effect of novel drug candidates. C57BL/6 mice tend to be resistant to Leishmania infections, whereas BALB/c mice display a susceptible phenotype. Studying species-specific interactions between Leishmania parasites and different host systems is a key step to characterize and validate these models for in vivo studies. Here, we use RNA-Seq and differential expression analysis to characterize the transcriptomic profiles of C57BL/6 and BALB/c peritoneal-derived macrophages in response to Leishmania panamensis infection. We observed differences between BALB/c and C57BL/6 macrophages regarding pathways associated with lysosomal degradation, arginine metabolism and the regulation of cell cycle. We also observed differences in the expression of chemokine and cytokine genes associated with regulation of immune responses. In conclusion, infection with L. panamensis induced an inflammatory gene expression pattern in C57BL/6 macrophages that is more consistently associated with a classic macrophage M1 activation, whereas in BALB/c macrophages a gene expression pattern consistent with an intermediate inflammatory response was observed.

Assuntos

Leishmaniose/metabolismo; Macrófagos Peritoneais/metabolismo; Transcriptoma; Animais; Modelos Animais de Doenças; Feminino; Mediadores da Inflamação; Leishmania guyanensis/fisiologia; Leishmaniose/genética; Macrófagos Peritoneais/parasitologia; Camundongos Endogâmicos BALB C; Camundongos Endogâmicos C57BL; RNA-Seq

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmaniose / Macrófagos Peritoneais / Transcriptoma Tipo de estudo: Risk_factors_studies Limite: Animals País/Região como assunto: America central / Panama Idioma: En Revista: PLoS Negl Trop Dis Assunto da revista: MEDICINA TROPICAL Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Panamá País de publicação: Estados Unidos

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google