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Altered non-coding RNA expression profile in F1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish.
Martín, Leonardo; Kamstra, Jorke H; Hurem, Selma; Lindeman, Leif C; Brede, Dag A; Aanes, Håvard; Babiak, Igor; Arenal, Amilcar; Oughton, Deborah; Salbu, Brit; Lyche, Jan Ludvig; Aleström, Peter.
Afiliação
  • Martín L; Morphophysiology Department, Faculty of Agricultural Sciences, University of Camagüey Ignacio Agramonte y Loynaz, 74 650, Camagüey, Cuba. leonardo.martin@reduc.edu.cu.
  • Kamstra JH; CERAD CoE, Department of Paraclinical Sciences, Norwegian University of Life Sciences, P.O. Box 5003, Ås, Norway. leonardo.martin@reduc.edu.cu.
  • Hurem S; CERAD CoE, Department of Paraclinical Sciences, Norwegian University of Life Sciences, P.O. Box 5003, Ås, Norway.
  • Lindeman LC; Institute for Risk Assessment Sciences (IRAS), Utrecht University, Utrecht, The Netherlands.
  • Brede DA; CERAD CoE, Department of Paraclinical Sciences, Norwegian University of Life Sciences, P.O. Box 5003, Ås, Norway.
  • Aanes H; Department of Paraclinical Sciences, Norwegian University of Life Sciences, 0454, Oslo, Norway.
  • Babiak I; CERAD CoE, Department of Paraclinical Sciences, Norwegian University of Life Sciences, P.O. Box 5003, Ås, Norway.
  • Arenal A; Department of Preclinical Sciences and Pathology, Norwegian University of Life Sciences, 0454, Oslo, Norway.
  • Oughton D; CERAD CoE, Department of Paraclinical Sciences, Norwegian University of Life Sciences, P.O. Box 5003, Ås, Norway.
  • Salbu B; Department of Environmental Science, Norwegian University of Life Sciences, 1433, Ås, Norway.
  • Lyche JL; PatoGen AS, P.O.box 548, 6001, Ålesund, Norway.
  • Aleström P; Faculty of Biosciences and Aquaculture, Nord University, 8026, Bodø, Norway.
Sci Rep ; 11(1): 4142, 2021 02 18.
Article em En | MEDLINE | ID: mdl-33602989
Gamma radiation produces DNA instability and impaired phenotype. Previously, we observed negative effects on phenotype, DNA methylation, and gene expression profiles, in offspring of zebrafish exposed to gamma radiation during gametogenesis. We hypothesize that previously observed effects are accompanied with changes in the expression profile of non-coding RNAs, inherited by next generations. Non-coding RNA expression profile was analysed in F1 offspring (5.5 h post-fertilization) by high-throughput sequencing 1 year after parental irradiation (8.7 mGy/h, 5.2 Gy total dose). Using our previous F1-γ genome-wide gene expression data (GSE98539), hundreds of mRNAs were predicted as targets of differentially expressed (DE) miRNAs, involved in pathways such as insulin receptor, NFkB and PTEN signalling, linking to apoptosis and cancer. snRNAs belonging to the five major spliceosomal snRNAs were down-regulated in the F1-γ group, Indicating transcriptional and post-transcriptional alterations. In addition, DEpiRNA clusters were associated to 9 transposable elements (TEs) (LTR, LINE, and TIR) (p = 0.0024), probable as a response to the activation of these TEs. Moreover, the expression of the lincRNAs malat-1, and several others was altered in the offspring F1, in concordance with previously observed phenotypical alterations. In conclusion, our results demonstrate diverse gamma radiation-induced alterations in the ncRNA profiles of F1 offspring observable 1 year after parental irradiation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peixe-Zebra / RNA não Traduzido / Raios gama Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Cuba País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peixe-Zebra / RNA não Traduzido / Raios gama Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Cuba País de publicação: Reino Unido