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Assessment of the effects of exposure to extremely low-frequency magnetic fields on MDCK epithelial cell lines under a controlled environment.
Domínguez, Gonzalo; Cardiel, Eladio; Sánchez, Elsa; Hernández, Pablo-Rogelio.
Afiliação
  • Domínguez G; Department of Electrical Engineering, Bioelectronics section, Center for Research and Advanced Studies of the National Polytechnic Institute, Mexico City, Mexico.
  • Cardiel E; Department of Electrical Engineering, Bioelectronics section, Center for Research and Advanced Studies of the National Polytechnic Institute, Mexico City, Mexico.
  • Sánchez E; Department of Physiology, Biophysics, and Neuroscience, Center for Research and Advanced Studies of the National Polytechnic Institute, Mexico City, Mexico.
  • Hernández PR; Department of Electrical Engineering, Bioelectronics section, Center for Research and Advanced Studies of the National Polytechnic Institute, Mexico City, Mexico.
J Radiat Res ; 62(2): 259-268, 2021 Mar 10.
Article em En | MEDLINE | ID: mdl-33592097
To assess the effects of exposure to extremely low-frequency magnetic fields (ELF-MFs) on MDCK cell lines, experiments were performed in a chamber under controlled conditions (temperature, humidity and CO2). Therefore, the measured physicochemical and electrical changes in the cells are due solely to the magnetic field exposure and not to external factors. A developed sinusoidal magnetic field generator produced the ELF-MFs with a uniform magnetic field and adjustable intensity and frequency. Three experimental indicators were used: (i) transepithelial electrical impedance (TEEI); (ii) cell migration and proliferation; and (iii) expression of the proteins of the tight junctions, and changes in the area and shape of the cell nuclei. No significant effects on TEEI values were observed when 10 and 50 G 60 Hz magnetic fields were applied to confluent cell monolayers. There were no significant differences in migration and proliferation of the cell monolayer exposed to 60 Hz magnetic fields10 and 50 G , but a contact inhibition factor was observed. The expression of the CLDN-1 protein decreased by 90% compared with the control, while ZO-1 protein expression increased by 120%. No significant effects were observed in the area and shape of the cell nuclei. Experimentation in a controlled environment, under physiological conditions, ensures that the observed effects were strictly due to exposure to magnetic fields. Different exposure conditions are necessary to determine the impact on TEEI and cell migration-proliferation indicators.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ambiente Controlado / Células Epiteliais / Campos Magnéticos Limite: Animals Idioma: En Revista: J Radiat Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ambiente Controlado / Células Epiteliais / Campos Magnéticos Limite: Animals Idioma: En Revista: J Radiat Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido