Your browser doesn't support javascript.
loading
Post-weaning protein malnutrition induces myocardial dysfunction associated with oxidative stress and altered calcium handling proteins in adult rats.
Belchior, Aucélia Cristina Soares; Freire-Júnior, David Domingues; Da Costa, Carlos Peres; Vassallo, Dalton Valentim; Dos Santos, Leonardo; Padilha, Alessandra Simão.
Afiliação
  • Belchior ACS; Departament of Physiology and Pharmacology, Federal University of Pernambuco, Recife, PE, Brazil.
  • Freire-Júnior DD; Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria,, ES, Brazil.
  • Da Costa CP; Departament of Physiology and Pharmacology, Federal University of Pernambuco, Recife, PE, Brazil.
  • Vassallo DV; Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria,, ES, Brazil.
  • Dos Santos L; Escola de Ensino Superior da Santa Casa de Misericórdia de Vitória, EMESCAM, Vitoria,, ES, Brazil.
  • Padilha AS; Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria,, ES, Brazil. leodossantos@hotmail.com.
J Physiol Biochem ; 77(2): 261-272, 2021 May.
Article em En | MEDLINE | ID: mdl-33555533
Hypercaloric low-protein diet may lead to a state of malnutrition found in the low-income population of Northeastern Brazil. Although malnutrition during critical periods in the early life is associated with cardiovascular diseases in adulthood, the mechanisms of cardiac dysfunction are still unclear. Here we studied the effects of post-weaning malnutrition due to low protein intake induced by a regional basic diet on the cardiac contractility of young adult rats. In vivo arterial hemodynamic and in vitro myocardial contractility were evaluated in 3-month-old rats. Additionally, protein content of the sarcoplasmic reticulum Ca(2+)-ATPase (SERCA), total phospholamban (PLB) and phosphorylated at serine 16 (p-Ser(16)-PLB), α2-subunit of the Na(+)/K(+)-ATPase (α2-NKA), and Na(+)/Ca(2+) exchanger (NXC) and in situ production of superoxide anion (O2(-)) were measured in the heart. Blood pressure and heart rate increased in the post-weaning malnourished (PWM) rats. Moreover, malnutrition decreased twitch force and inotropic responses of the isolated cardiac muscle. Protein expression of SERCA, PLB/SERCA, and p-Ser(16)-PLB/PLB ratios and α2-NKA were decreased without changing NCX. The contraction dependent on transsarcolemmal calcium influx was unchanged but responsiveness to Ca(2+) and tetanic peak contractions were impaired in the PWM group. Myocardial O2(-) production was significantly increased by PWM. Our data demonstrated that this hypercaloric low-protein diet in rats is associated with myocardial dysfunction, altered expression of major calcium handling proteins, and increased local oxidative stress. These findings reinforce the attention needed for pediatric care, since chronic malnutrition in early life is related to increased cardiovascular risk in adulthood. Graphical Abstract.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cálcio / Desnutrição Proteico-Calórica / Dieta com Restrição de Proteínas / Trocador de Sódio e Cálcio / Miocárdio Tipo de estudo: Etiology_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: J Physiol Biochem Assunto da revista: BIOQUIMICA / FISIOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Espanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cálcio / Desnutrição Proteico-Calórica / Dieta com Restrição de Proteínas / Trocador de Sódio e Cálcio / Miocárdio Tipo de estudo: Etiology_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: J Physiol Biochem Assunto da revista: BIOQUIMICA / FISIOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Espanha