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Infrared Spectroscopy Based Study of Biochemical Changes in Saliva during Maximal Progressive Test in Athletes.
Vieira, Claudio A G A; Pupin, Breno; Bhattacharjee, Tanmoy T; Sakane, Kumiko K.
Afiliação
  • Vieira CAGA; Infrared Spectroscopy Laboratory, Research and Development Institute R&DI, University of Vale do Paraíba-Univap, Shishima Hifumi Avenue, 2911, 12244-000, São Jose dos Campos, São Paulo, Brazil.
  • Pupin B; Infrared Spectroscopy Laboratory, Research and Development Institute R&DI, University of Vale do Paraíba-Univap, Shishima Hifumi Avenue, 2911, 12244-000, São Jose dos Campos, São Paulo, Brazil.
  • Bhattacharjee TT; Sir John Walsh Research Institute, University of Otago, 310 Great King St, North Dunedin, Dunedin, 9016, New Zealand. tanmoytbhattacharjee@gmail.com.
  • Sakane KK; Infrared Spectroscopy Laboratory, Research and Development Institute R&DI, University of Vale do Paraíba-Univap, Shishima Hifumi Avenue, 2911, 12244-000, São Jose dos Campos, São Paulo, Brazil.
Anal Sci ; 37(8): 1157-1163, 2021 Aug 10.
Article em En | MEDLINE | ID: mdl-33518584
This study aims to explore biochemical changes in saliva during cardiorespiratory exercise using attenuated-total-reflectance-Fourier-transform-infrared-spectroscopy (ATR-FTIR). Saliva and blood samples were obtained from six athletes at rest, and after running at speeds of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, and 20 kilometers-per-hour (km/h) on a treadmill (maximal stress test). Saliva ATR-FTIR spectra were analyzed using deconvolution and multivariate analysis. Area-under-the-curve calculations suggest differential changes in glucose, lactate, protein, lipids, carbohydrate and phosphate content in saliva during the test. Increases in glucose and lactate levels with increasing speeds were verified by simultaneous measurement of blood glucose and lactate levels using standard equipment (Roche®). Multivariate principal-component-analysis (PCA) showed discrete clusters for low (rest-14 km/h) and high (15 - 20 km/h) speeds, and PCA-linear-discriminant-analysis showed 100% classification of 18 - 20 km/h as high speed. Overall, results suggest the possibility of using this non-invasive saliva-based ATR-FTIR method for biochemical assessment during sports exercise and stress tests.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saliva / Atletas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Anal Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saliva / Atletas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Anal Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça