Novel Genetic Constructs for Production of Recombinant HTLV-1/2 Antigens and Evaluation of Their Reactivity to Plasma Samples from HTLV-1-Infected Patients.

Oliveira, Ueriton Dias de; Santos, Fred Luciano Neves; Galvão-Castro, Bernardo; Krieger, Marco Aurelio; Zanchin, Nilson Ivo Tonin

Oliveira, Ueriton Dias de; Santos, Fred Luciano Neves; Galvão-Castro, Bernardo; Krieger, Marco Aurelio; Zanchin, Nilson Ivo Tonin.

Afiliação

Oliveira UD; Laboratory of Structural Biology and Protein Engineering, Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Paraná, Brazil.
Santos FLN; Cellular and Molecular Biology Postgraduate Program, Federal University of Paraná, Curitiba, Paraná, Brazil.
Galvão-Castro B; Advanced Public Health Laboratory, Gonçalo Moniz Institute, Fiocruz, Salvador, Bahia, Brazil.
Krieger MA; Advanced Public Health Laboratory, Gonçalo Moniz Institute, Fiocruz, Salvador, Bahia, Brazil.
Zanchin NIT; Integrated and Multidisciplinary HTLV Center, Bahiana School of Medicine and Public Health, Salvador, Bahia, Brazil.

J Clin Microbiol ; 59(4)2021 03 19.

Article em En | MEDLINE | ID: mdl-33504592

RESUMO

Human T-cell leukemia virus type 1 (HTLV-1) can cause life-threatening diseases for which there are no effective treatments. Prevention of HTLV-1 infection requires massive testing of pregnant women, blood for transfusion, and organs for transplantation, as well as safe sex. In this context, serological assays are widely used for monitoring HTLV-1 infections. Despite the necessity for recombinant antigens to compose serological tests, there is little information available on procedures to produce recombinant HTLV-1/2 antigens for serological diagnostic purposes. In this work, we tested a series of genetic constructions to select those more amenable for production in bacterial systems. To overcome the constraints in expressing sections of viral envelope proteins in bacteria, we have used the p24 segment of the gag protein as a scaffold to display the immunogenic regions of gp46 and gp21. Nine recombinant antigenic proteins derived from HTLV-1 and five derived from HTLV-2 were successfully purified. The HTLV-1 antigens showed high efficiency in discriminating HTLV-positive samples from HTLV-negative samples using enzyme-linked immunosorbent assays. Interestingly, HTLV-1-positive samples showed a high level of cross-reaction with HTLV-2 antigens. This finding is explained by the high sequence conservation between the structural proteins of these two highly related viruses. In summary, the results presented in this work provide a detailed description of the methods used to produce recombinant HTLV-1 and HTLV-2 antigens, and they demonstrate that the HTLV-1 antigens show strong potential for serological diagnosis of HTLV-1 infections.

Assuntos

Infecções por HTLV-I; Vírus Linfotrópico T Tipo 1 Humano; Ensaio de Imunoadsorção Enzimática; Feminino; Produtos do Gene gag/genética; Infecções por HTLV-I/diagnóstico; Vírus Linfotrópico T Tipo 1 Humano/genética; Vírus Linfotrópico T Tipo 2 Humano/genética; Humanos; Gravidez

Palavras-chave

ELISA; human T-cell leukemia virus; immunoassays; recombinant-protein production

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus Linfotrópico T Tipo 1 Humano / Infecções por HTLV-I Tipo de estudo: Diagnostic_studies Limite: Female / Humans / Pregnancy Idioma: En Revista: J Clin Microbiol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

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