Your browser doesn't support javascript.
loading
Cytotoxic and genotoxic effects in human gingival fibroblast and ions release of endodontic sealers incorporated with nanostructured silver vanadate.
Teixeira, Ana B V; Moreira, Natália C S; Takahashi, Catarina S; Schiavon, Marco A; Alves, Oswaldo L; Reis, Andréa C.
Afiliação
  • Teixeira ABV; Departament of Dental Materials and Prosthesis, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, Brazil.
  • Moreira NCS; Departament of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
  • Takahashi CS; Departament of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
  • Schiavon MA; Departament of Natural Sciences, Federal University of São João Del-Rei, São João Del-Rei, Brazil.
  • Alves OL; Laboratory of Solid State Chemistry, Institute of Chemistry, University of Campinas (Unicamp), Campinas, Brazil.
  • Reis AC; Departament of Dental Materials and Prosthesis, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, Brazil.
J Biomed Mater Res B Appl Biomater ; 109(9): 1380-1388, 2021 09.
Article em En | MEDLINE | ID: mdl-33470054
The cytotoxic and genotoxic effects of commercial endodontic sealers (AH Plus, Sealer 26 and Endomethasone N) incorporated with nanostructured silver vanadate decorated with silver nanoparticles (AgVO3 - at concentrations 2.5, 5, and 10%) on human gingival fibroblast (HGF), and the silver (Ag+ ) and vanadium (V4+ /V5+ ) ions release were evaluated. Cytotoxicity, cell death, and genotoxicity tests were carried out with extract samples of 24-hr and 7-days. The release of Ag+ and V4+ /V5+ was evaluated. Cytotoxicity in HGF was caused by AH Plus (AP) with 5 and 10% of AgVO3 (83.84 and 67.49% cell viability, respectively) with 24-hr extract (p < 0.05), as well as all concentrations of AP with 7-days extract (p < 0.05 -AP 0% = 73.17%; AP 2.5% = 75.07%; AP 5% = 70.62%; AP 10% = 68.46% cell viability). The commercial sealers Sealer 26 (S26) and Endomethasone N (EN) were cytotoxic (p < 0.05 - S26 0% = 34.81%; EN 0% = 20.99% cell viability with 7-days extract). AP 10% with 7-days extract induced 32% apoptotic cells in HGF (p < 0.05). Genotoxic effect was not observed. The AP groups released more Ag+ , while S26 and EN released more V4+ /V5+ in 24 hr. The Ag+ can be cytotoxic. In conclusion, the cytotoxicity caused to HGF can be attributed by the commercial sealers and enhanced by incorporation of AgVO3 , was not observed genotoxic effect, and apoptosis was induced only by AH Plus 10% 7-days extract. Ag+ can influence cell viability.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Materiais Restauradores do Canal Radicular / Prata / Vanádio / Bismuto / Hidróxido de Cálcio / Fibroblastos / Gengiva / Antibacterianos Limite: Humans Idioma: En Revista: J Biomed Mater Res B Appl Biomater Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Materiais Restauradores do Canal Radicular / Prata / Vanádio / Bismuto / Hidróxido de Cálcio / Fibroblastos / Gengiva / Antibacterianos Limite: Humans Idioma: En Revista: J Biomed Mater Res B Appl Biomater Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos