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Dietary Fat Quality in Normolipidic Diets Affects Hepatocyte's Nuclear Phenotypes.
Barbutti, Isabella; Laurentino, Jesse C; Va da Silva, Natalia; Deoclécio, Vinicius S; Ferrucci, Danilo; Carvalho, Hernandes F.
Afiliação
  • Barbutti I; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, São Paulo, Brazil.
  • Laurentino JC; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, São Paulo, Brazil.
  • Va da Silva N; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, São Paulo, Brazil.
  • Deoclécio VS; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, São Paulo, Brazil.
  • Ferrucci D; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, São Paulo, Brazil.
  • Carvalho HF; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, São Paulo, Brazil.
Nutr Metab Insights ; 13: 1178638820982003, 2020.
Article em En | MEDLINE | ID: mdl-33414640
Dietary fat quality affects overall systemic parameters and produce hepatic accumulation of fat and inflammation (steatohepatitis). In this communication we have assessed how mouse liver nuclear phenotypes are influenced by diets containing 7% lipid prepared with lard, linseed oil or soybean oil for 32 weeks. Liver specimens were imprinted on glass slides, fixed and stained with DAPI. 3D confocal images were obtained and employed for the calculation of nuclear thickness, nuclear volume and DAPI-DNA intensity. Hepatocytes' nuclei could be classified as diploid A, diploid B, tetraploid and higher ploidy levels. Linseed oil in the diet resulted in increased frequency of diploid A (more compact) and less polyploidy, while lard caused increased volume and more polyploidy. Soybean oil produced intermediate nuclear sizes. The results suggest a high demand on liver physiology promoted by lard, which has a predominance of saturated fatty acids, while linseed oil promoted the opposite effect.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Nutr Metab Insights Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Nutr Metab Insights Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos