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[Carbapenemases in Pseudomonas aeruginosa with decreased susceptibility to carbapenems after a decade: from VIM to KPC]. / Carbapenemasas en Pseudomonas aeruginosa con susceptibilidad disminuida a los carbapenémicos después de una década, desde VIM a KPC.
Gaete, Margarita Enberg; Valenzuela, Marcela Puente; Bachero, Aniela Wozniak; Vega, Claudia Castillo; Marín, Nicolás Villagra; Labarca, Jaime Labarca; Cañete, Patricia García.
Afiliação
  • Gaete ME; Departamento de Enfermedades Infecciosas del Adulto, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Valenzuela MP; Departamento de Enfermedades Infecciosas del Adulto, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Bachero AW; Departamento de Laboratorios Clínicos, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Vega CC; Laboratorio de Microbiología, Servicio de Laboratorios Clínicos, Red de Salud UC-CHRISTUS, Santiago, Chile.
  • Marín NV; Departamento de Laboratorios Clínicos, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Labarca JL; Departamento de Enfermedades Infecciosas del Adulto, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Cañete PG; Departamento de Laboratorios Clínicos, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
Rev Chilena Infectol ; 37(4): 389-394, 2020 Aug.
Article em Es | MEDLINE | ID: mdl-33399659
BACKGROUND: Pseudomonas aeruginosa is a relevant infectious agent affecting patients within health care setting; this situation is worsening with the appearance of strains resistance to carbapenems. AIMS: To study carbapenemase production in P. aeruginosa with decreased susceptibility to carbapenems processed in the microbiology laboratory of the Health Network UC-CHRISTUS in 2014-2015 and compare them with the strains studied in 2004-2005. METHODS: Between January 2014 and June 2015, 459 strains of P. aeruginosa from clinical samples were isolated. Susceptibility was determined by dilution in agar and strains with reduced susceptibility to carbapenems were tested for carbaNP. Positive strains were studied by PCR for blaVIM, blaVIM-1, blaVIM-2, blaIMP, blaNDM, blaKPC, blaOXA and blaIMI genes. Pulsed field electrophoresis was performed on selected strains. RESULTS: From 459 strains studied, 300 had reduced susceptibility to carbapenems (65.3%). Of these, 183 were viable for study, corresponding to 164 patients. The carbaNP test was positive in 44 strains of the 183 strains (24%). The resistance genes found were: blaVIM-2 in 35 strains, blaKPC-2+VIM-2 in 7 strains and blaKPC-2 in 2 strains. In the blaKPC-2 strains clonal relation between them was found. CONCLUSIONS: A 65.3% of P. aeruginosa presented decreased susceptibility to carbapenems being the presence of carbapenemases not the main resistance mechanism. In addition, the emergence in Chile of P. aeruginosa strains with bla of the KPC-2 type alone or in combination with VIM-2 is described.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Carbapenêmicos Limite: Humans País/Região como assunto: America do sul / Chile Idioma: Es Revista: Rev Chilena Infectol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile País de publicação: Chile
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Carbapenêmicos Limite: Humans País/Região como assunto: America do sul / Chile Idioma: Es Revista: Rev Chilena Infectol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile País de publicação: Chile