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Complexin-2 redistributes to the membrane of muscle cells in response to insulin and contributes to GLUT4 translocation.
Pavarotti, Martin Alejandro; Tokarz, Victoria; Frendo-Cumbo, Scott; Bilan, Philip J; Liu, Zhi; Zanni-Ruiz, Emilia; Mayorga, Luis Segundo; Klip, Amira.
Afiliação
  • Pavarotti MA; Laboratorio de Fusión de Membranas y Exocitosis Acrosomal, Instituto de Histología y Embriología Mendoza Dr. Mario H Burgos, Mendoza, Argentina.
  • Tokarz V; Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
  • Frendo-Cumbo S; Universidad Nacional de Cuyo, Mendoza, Argentina.
  • Bilan PJ; Cell Biology Program, Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
  • Liu Z; Cell Biology Program, Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
  • Zanni-Ruiz E; Cell Biology Program, Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
  • Mayorga LS; Cell Biology Program, Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
  • Klip A; Laboratorio de Fusión de Membranas y Exocitosis Acrosomal, Instituto de Histología y Embriología Mendoza Dr. Mario H Burgos, Mendoza, Argentina.
Biochem J ; 478(2): 407-422, 2021 01 29.
Article em En | MEDLINE | ID: mdl-33393983
Insulin stimulates glucose uptake in muscle cells by rapidly redistributing vesicles containing GLUT4 glucose transporters from intracellular compartments to the plasma membrane (PM). GLUT4 vesicle fusion requires the formation of SNARE complexes between vesicular VAMP and PM syntaxin4 and SNAP23. SNARE accessory proteins usually regulate vesicle fusion processes. Complexins aide in neuro-secretory vesicle-membrane fusion by stabilizing trans-SNARE complexes but their participation in GLUT4 vesicle fusion is unknown. We report that complexin-2 is expressed and homogeneously distributed in L6 rat skeletal muscle cells. Upon insulin stimulation, a cohort of complexin-2 redistributes to the PM. Complexin-2 knockdown markedly inhibited GLUT4 translocation without affecting proximal insulin signalling of Akt/PKB phosphorylation and actin fiber remodelling. Similarly, complexin-2 overexpression decreased maximal GLUT4 translocation suggesting that the concentration of complexin-2 is finely tuned to vesicle fusion. These findings reveal an insulin-dependent regulation of GLUT4 insertion into the PM involving complexin-2.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Mioblastos / Proteínas Adaptadoras de Transporte Vesicular / Transportador de Glucose Tipo 4 / Insulina / Proteínas do Tecido Nervoso Limite: Animals Idioma: En Revista: Biochem J Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Mioblastos / Proteínas Adaptadoras de Transporte Vesicular / Transportador de Glucose Tipo 4 / Insulina / Proteínas do Tecido Nervoso Limite: Animals Idioma: En Revista: Biochem J Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido