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Electrical stimulation enhances early palatal wound healing in mice.
Ferreira, Camila Lopes; Neves Jardini, Maria Aparecida; Moretto Nunes, Camilla Magnoni; Bernardo, Daniella Vicensotto; Viana Casarin, Renato Corrêa; Dos Santos Gedraite, Estevão; Mathias, Márcio Antônio; Liu, Fei; Mendonça, Gustavo; Silveira Mendonça, Daniela Baccelli; Santamaria, Mauro Pedrine.
Afiliação
  • Ferreira CL; Postgraduate Student, Oral Biopathology Program, Periodontics Area, São Paulo State University (UNESP), College of Dentistry, São José dos Campos, Brazil.
  • Neves Jardini MA; São Paulo State University (UNESP), Division of Periodontics, Institute of Science and Technology, São José dos Campos, São Paulo, Brazil.
  • Moretto Nunes CM; Postgraduate Student, Oral Biopathology Program, Periodontics Area, São Paulo State University (UNESP), College of Dentistry, São José dos Campos, Brazil.
  • Bernardo DV; Postgraduate Student, Oral Biopathology Program, Periodontics Area, São Paulo State University (UNESP), College of Dentistry, São José dos Campos, Brazil.
  • Viana Casarin RC; University of Campinas (UNICAMP). Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, Piracicaba, São Paulo, Brazil.
  • Dos Santos Gedraite E; FEI - College of Industrial Engineering, São Bernardo do Campo, São Paulo, Brazil.
  • Mathias MA; FEI - College of Industrial Engineering, São Bernardo do Campo, São Paulo, Brazil.
  • Liu F; University of Michigan, School of Dentistry, Department of Biologic and Materials Sciences & Prosthodontics, Ann Arbor, USA.
  • Mendonça G; University of Michigan, School of Dentistry, Department of Biologic and Materials Sciences & Prosthodontics, Ann Arbor, USA.
  • Silveira Mendonça DB; University of Michigan, School of Dentistry, Department of Biologic and Materials Sciences & Prosthodontics, Ann Arbor, USA.
  • Santamaria MP; São Paulo State University (UNESP), Division of Periodontics, Institute of Science and Technology, São José dos Campos, São Paulo, Brazil. Electronic address: mauro.santamaria@unesp.br.
Arch Oral Biol ; 122: 105028, 2021 Feb.
Article em En | MEDLINE | ID: mdl-33360374
BACKGROUND/OBJECTIVE: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. METHODS: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 µA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1ß, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. RESULTS: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. CONCLUSION: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Palato / Cicatrização / Ferimentos e Lesões / Estimulação Elétrica / Mucosa Bucal Limite: Animals Idioma: En Revista: Arch Oral Biol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Palato / Cicatrização / Ferimentos e Lesões / Estimulação Elétrica / Mucosa Bucal Limite: Animals Idioma: En Revista: Arch Oral Biol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido