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Vitamin D status influences cytokine production and MALAT1 expression from the PBMCs of patients with coronary artery disease and healthy controls.
Nowrouzi-Sohrabi, Peyman; Kalani, Mehdi; Izadpanah, Peyman; Ahmadvand, Hassan; Fakhour, Masoumeh; Fadaei, Reza; Khorshidifar, Meghdad; Seghatoleslam, Atefeh.
Afiliação
  • Nowrouzi-Sohrabi P; Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Kalani M; Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Izadpanah P; Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Ahmadvand H; Cardiology Department, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Fakhour M; Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
  • Fadaei R; Razi Herbal Researches Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
  • Khorshidifar M; Faculty of Allied Medical Sciences, Zabol University of Medical Sciences, Zabol, Iran.
  • Seghatoleslam A; Sleep Disorders Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Rev Assoc Med Bras (1992) ; 66(12): 1712-1717, 2020 Dec.
Article em En | MEDLINE | ID: mdl-33331582
OBJECTIVE: This study aimed to investigate the long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (lncRNA MALAT1) expression and its role in cytokine production from peripheral blood mononuclear cells (PBMCs) in patients with coronary artery disease (CAD) and non-CAD participants (NCAD). METHODS: Blood samples were taken from 15 patients with CAD and 15 NCAD individuals. The plasma was used for biochemical analyses. MALAT1 and CD36 expressions were evaluated in the isolated peripheral blood mononuclear cells (PBMCs) by real-time PCR. Furthermore, the levels of inflammatory cytokines e.g. interleukin (IL)-6, IL-10, and IL-22 were measured in the supernatants of the cultured PBMCs by flow cytometry. RESULTS: The levels of MALAT1 and CD36 were not significantly different between the CAD and NCAD groups. However, a lower level of MALAT1 and CD36 was observed in PBMCs of vitamin D deficient (<15 ng/ml) CAD and NCAD participants. Furthermore, the vitamin D deficient (<15 ng/ml) group showed a significantly higher plasma level of IL-6, IL-10, and IL-22 compared to the non-deficient (≥15 ng/ml) group. In addition, significant positive correlations were found between CD36, IL-22, and fasting blood sugar (FBS) with MALAT1. CONCLUSION: Given that in vitamin D deficient individuals a decreased level of MALAT1 was associated with CD36 expression and increased IL-22 production, vitamin D supplementation may play a role in reducing MALAT1/CD36/IL-22 mediated complications such as T2DM and CAD, especially in vitamin D deficiency.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença da Artéria Coronariana / RNA Longo não Codificante Limite: Humans Idioma: En Revista: Rev Assoc Med Bras (1992) Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença da Artéria Coronariana / RNA Longo não Codificante Limite: Humans Idioma: En Revista: Rev Assoc Med Bras (1992) Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil