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The role of glucose metabolism and insulin resistance in cardiac remodelling induced by cigarette smoke exposure.
Azevedo, Paula Schmidt; Polegato, Bertha F; Paiva, Sergio; Costa, Nara; Santos, Priscila; Bazan, Silmeia; Fernandes, Ana Angelica Henrique; Fabro, Alexandre; Pires, Vanessa; Tanni, Suzana E; Leal Pereira, Filipe; Lo, Angelo; Grassi, Leticia; Campos, Dijon; Androcioli, Vickeline; Zornoff, Leonardo; Minicucci, Marcos.
Afiliação
  • Azevedo PS; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Polegato BF; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Paiva S; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Costa N; Faculty of Nutrition, UFG - Univ Federal de Goiás, Goiânia, Brazil.
  • Santos P; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Bazan S; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Fernandes AAH; Department of Chemical and Biological Sciences, São Paulo State University-UNESP, Botucatu, Brazil.
  • Fabro A; Department of Pathology and Legal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
  • Pires V; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Tanni SE; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Leal Pereira F; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Lo A; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Grassi L; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Campos D; Department of Pathology and Legal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
  • Androcioli V; Experimental Research Unit - UNIPEX, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Zornoff L; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
  • Minicucci M; Department of Internal Medicine, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.
J Cell Mol Med ; 25(2): 1314-1318, 2021 01.
Article em En | MEDLINE | ID: mdl-33300293
The aim of this study is to evaluate whether the alterations in glucose metabolism and insulin resistance are mechanisms presented in cardiac remodelling induced by the toxicity of cigarette smoke. Male Wistar rats were assigned to the control group (C; n = 12) and the cigarette smoke-exposed group (exposed to cigarette smoke over 2 months) (CS; n = 12). Transthoracic echocardiography, blood pressure assessment, serum biochemical analyses for catecholamines and cotinine, energy metabolism enzymes activities assay; HOMA index (homeostatic model assessment); immunohistochemistry; and Western blot for proteins involved in energy metabolism were performed. The CS group presented concentric hypertrophy, systolic and diastolic dysfunction, and higher oxidative stress. It was observed changes in energy metabolism, characterized by a higher HOMA index, lower concentration of GLUT4 (glucose transporter 4) and lower 3-hydroxyl-CoA dehydrogenase activity, suggesting the presence of insulin resistance. Yet, the cardiac glycogen was depleted, phosphofructokinase (PFK) and lactate dehydrogenase (LDH) increased, with normal pyruvate dehydrogenase (PDH) activity. The activity of citrate synthase, mitochondrial complexes and ATP synthase (adenosine triphosphate synthase) decreased and the expression of Sirtuin 1 (SIRT1) increased. In conclusion, exposure to cigarette smoke induces cardiac remodelling and dysfunction. The mitochondrial dysfunction and heart damage induced by cigarette smoke exposure are associated with insulin resistance and glucose metabolism changes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resistência à Insulina / Fumar / Remodelação Ventricular / Glucose Limite: Animals Idioma: En Revista: J Cell Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resistência à Insulina / Fumar / Remodelação Ventricular / Glucose Limite: Animals Idioma: En Revista: J Cell Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido