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Physical exercise stimulates hippocampal mTORC1 and FNDC5/irisin signaling pathway in mice: Possible implication for its antidepressant effect.
Gruhn, Karen; Siteneski, Aline; Camargo, Anderson; Freitas, Andiara E; Olescowicz, Gislaine; Brocardo, Patricia S; Rodrigues, Ana Lúcia S.
Afiliação
  • Gruhn K; Department of Biochemistry, Center of Biological Sciences, Federal University of Santa Catarina, 88040-900, Florianópolis, Santa Catarina, Brazil.
  • Siteneski A; Department of Biochemistry, Center of Biological Sciences, Federal University of Santa Catarina, 88040-900, Florianópolis, Santa Catarina, Brazil; Institute of Investigation, Faculty of Health Sciences, Technical University of Manabí, 130103, Portoviejo, Manabí, Ecuador.
  • Camargo A; Department of Biochemistry, Center of Biological Sciences, Federal University of Santa Catarina, 88040-900, Florianópolis, Santa Catarina, Brazil.
  • Freitas AE; Department of Biochemistry, Center of Biological Sciences, Federal University of Santa Catarina, 88040-900, Florianópolis, Santa Catarina, Brazil.
  • Olescowicz G; Department of Biochemistry, Center of Biological Sciences, Federal University of Santa Catarina, 88040-900, Florianópolis, Santa Catarina, Brazil.
  • Brocardo PS; Department of Morphological Sciences, Center of Biological Sciences, Federal University of Santa Catarina, 88040-900, Florianópolis, Santa Catarina, Brazil.
  • Rodrigues ALS; Department of Biochemistry, Center of Biological Sciences, Federal University of Santa Catarina, 88040-900, Florianópolis, Santa Catarina, Brazil. Electronic address: ana.l.rodrigues@ufsc.br.
Behav Brain Res ; 400: 113040, 2021 02 26.
Article em En | MEDLINE | ID: mdl-33279634
Several lines of evidence have consistently indicated that physical exercise has antidepressant effects by improving hippocampal function, although the signaling pathways underpinning these responses are not well established. Therefore, this study investigated the role of mechanistic target of rapamycin complex 1 (mTORC1) and fibronectin type III domain-containing protein 5 (FNDC5)/irisin signaling in the antidepressant-like effect of physical exercise. We showed that physical exercise (treadmill running - 45 min/day/5 days/week for 4 weeks) produced an antidepressant-like effect as indicated by a reduction on the immobility time in mice subjected to the forced swimming test (FST) without altering locomotor activity in the open field test (OFT). Rapamycin (a selective mTORC1 inhibitor, 0.2 nmol/site, i.c.v.) administration completely abolished the antidepressant-like effect of physical exercise in the FST, suggesting that mTORC1 activation plays a role for its behavioral effect. Accordingly, physical exercise increased the number of phosphorylated mTORC1 (Ser2448)-positive cells in the entire and ventral subgranular zone of the hippocampal dentate gyrus. Physical exercise was also effective in augmenting the hippocampal FNDC5/irisin immunocontent, but rapamycin administration did not alter this effect. Our results reinforce the notion that physical exercise exerts an antidepressant-like effect and identifies the mTORC1-mediated signaling pathway as a target for its behavioral effects. This study provides additional evidence that physical exercise increases hippocampal FNDC5/irisin immunocontent, but this effect seems to be independent on hippocampal mTORC1 activation. Altogether the results contribute to elucidate possible molecular targets implicated in the antidepressant effects of physical exercise and highlight the role of mTORC1 signaling for its behavioral response.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Condicionamento Físico Animal / Transdução de Sinais / Fibronectinas / Sirolimo / Alvo Mecanístico do Complexo 1 de Rapamicina / Hipocampo / Locomoção Limite: Animals Idioma: En Revista: Behav Brain Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Condicionamento Físico Animal / Transdução de Sinais / Fibronectinas / Sirolimo / Alvo Mecanístico do Complexo 1 de Rapamicina / Hipocampo / Locomoção Limite: Animals Idioma: En Revista: Behav Brain Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda