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Oral delivery of fish oil in oil-in-water nanoemulsion: development, colloidal stability and modulatory effect on in vivo inflammatory induction in mice.
Santos, Débora S; Morais, José Athayde V; Vanderlei, Ísis A C; Santos, Alexandre S; Azevedo, Ricardo B; Muehlmann, Luís A; Júnior, Osmindo R P; Mortari, Márcia R; da Silva, Jaqueline R; da Silva, Sebastião W; Longo, João P F.
Afiliação
  • Santos DS; Nanobiotechnology Laboratory, Genetics & Morphology Department, Institute of Biological Science, University of Brasilia, Brasília, 70910-900, Brazil.
  • Morais JAV; Nanobiotechnology Laboratory, Genetics & Morphology Department, Institute of Biological Science, University of Brasilia, Brasília, 70910-900, Brazil.
  • Vanderlei ÍAC; Neuropharmacology Laboratory, Physiological Sciences Department, Institute of Biological Science, University of Brasilia, Brasília, 70910-900, Brazil.
  • Santos AS; Optical Spectroscopy Laboratory, Institute of Physics, University of Brasilia, Brasília, 70910-900, Brazil.
  • Azevedo RB; Nanobiotechnology Laboratory, Genetics & Morphology Department, Institute of Biological Science, University of Brasilia, Brasília, 70910-900, Brazil.
  • Muehlmann LA; Nanobiotechnology Laboratory, Genetics & Morphology Department, Institute of Biological Science, University of Brasilia, Brasília, 70910-900, Brazil.
  • Júnior ORP; Toxinology Laboratory, Physiological Sciences Department, Institute of Biological Science, University of Brasilia, Brasília, 70910-900, Brazil.
  • Mortari MR; Neuropharmacology Laboratory, Physiological Sciences Department, Institute of Biological Science, University of Brasilia, Brasília, 70910-900, Brazil.
  • da Silva JR; Nanobiotechnology Laboratory, Genetics & Morphology Department, Institute of Biological Science, University of Brasilia, Brasília, 70910-900, Brazil.
  • da Silva SW; Optical Spectroscopy Laboratory, Institute of Physics, University of Brasilia, Brasília, 70910-900, Brazil.
  • Longo JPF; Nanobiotechnology Laboratory, Genetics & Morphology Department, Institute of Biological Science, University of Brasilia, Brasília, 70910-900, Brazil. Electronic address: jplongo82@gmail.com.
Biomed Pharmacother ; 133: 110980, 2021 Jan.
Article em En | MEDLINE | ID: mdl-33249282
To improve the oral absorption of fish oil and test its anti-inflammatory effect, a fish oil nanoemulsion was developed using cis-4,7,10,13,16,19-docosahexaenoic fatty acid as a biomarker for oral administration. The colloidal stability tests of the fish oil nanoemulsion showed an average size of 155.44 nm ±â€¯6.46 (4 °C); 163.04 nm ±â€¯9.97 (25 °C) and polydispersity index 0.22 ±â€¯0.02 (4 °C), 0.21 ±â€¯0.02 (25 °C), indicating systems with low polydispersity and stable droplets. The fish oil nanoemulsion did not alter the cell viability of the RAW 264.7 macrophages and, at a concentration of 0.024 mg/mL, was kinetically incorporated into the cells after 18 h of contact. The nanoemulsion was maintained in the gastrointestinal region for a significantly shorter period of time (p ≤ 0.05) compared to the intake of fish oil in free form. Inflammatory tests demonstrated that nanoemulsion and fish oil showed less (p ≤ 0.05) neutrophil infiltration after 24h of sepsis induction and there was a significant reduction (p ≤ 0.05) in the volume of paw edema in female adult Balb/c mice who received the nanoemulsion diet compared to the other experimental groups (control, formalin, fish oil and sunflower oil). These results indicate that the fish oil nanoemulsion was significantly effective in the dietary conditions tested here, presenting satisfactory responses in the modulation of inflammatory disorders, demonstrating interesting and beneficial nutraceutical effects.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Água / Ácido Eicosapentaenoico / Ácidos Docosa-Hexaenoicos / Edema / Nanopartículas / Inflamação / Anti-Inflamatórios Limite: Animals Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: França
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Água / Ácido Eicosapentaenoico / Ácidos Docosa-Hexaenoicos / Edema / Nanopartículas / Inflamação / Anti-Inflamatórios Limite: Animals Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: França