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Isolation of Harveyi clade Vibrio spp. collected in aquaculture farms: How can the identification issue be addressed?
Culot, Antoine; Grosset, Noel; Bruey, Quentin; Auzou, Michel; Giard, Jean-Christophe; Favard, Baptiste; Wakatsuki, Alexandre; Baron, Sandrine; Frouel, Stephane; Techer, Clarisse; Gautier, Michel.
Afiliação
  • Culot A; Microbiology Laboratory, Institut National de Recherche en Agriculture, Alimentation et Environnement, (INRAE), UMR 1253 Science and, Technology of Milk and Eggs (STLO), Rennes, France; Mixscience SAS, Bruz, France. Electronic address: a.culot@hotmail.com.
  • Grosset N; Microbiology Laboratory, Institut National de Recherche en Agriculture, Alimentation et Environnement, (INRAE), UMR 1253 Science and, Technology of Milk and Eggs (STLO), Rennes, France.
  • Bruey Q; Mixscience SAS, Bruz, France.
  • Auzou M; Service Microbiologie, Centre Hospitalier Universitaire de Caen, Caen, France.
  • Giard JC; Service Microbiologie, Centre Hospitalier Universitaire de Caen, Caen, France.
  • Favard B; Mixscience SAS, Bruz, France.
  • Wakatsuki A; Zoopeixes Fish Company, Jardim America, Goiânia, Brazil.
  • Baron S; Laboratoire de Ploufragan-Plouzane-Niort, ANSES, Ploufragan, France.
  • Frouel S; Mixscience SAS, Bruz, France.
  • Techer C; Mixscience SAS, Bruz, France.
  • Gautier M; Microbiology Laboratory, Institut National de Recherche en Agriculture, Alimentation et Environnement, (INRAE), UMR 1253 Science and, Technology of Milk and Eggs (STLO), Rennes, France. Electronic address: michel.gautier@agrocampus-ouest.fr.
J Microbiol Methods ; 180: 106106, 2021 01.
Article em En | MEDLINE | ID: mdl-33248180
Aquaculture is a fast growing industry with its development hampered by bacterial diseases. Vibriosis caused by Harveyi clade strains is known for causing heavy loss especially in shrimp aquaculture farms. For farm treatment and pathogen spread management, veterinarians and researchers need reliable bacterial identification tools. A range of identification methods have been presented for Vibrio spp. in recent literature but little feedback on their performance have been made available to this day. This study aims at comparing Vibrio spp. identification methods and providing guidance on their use. Fifty farms were sampled and bacterial colonies were isolated using specific culture media before microscopic analysis and genomic profiling using ERIC-PCR. A preliminary identification step was carried out using MALDI-ToF mass spectrometry. Four methods were compared for strain identification on 14 newly isolated Harveyi clade Vibrio spp. strains: whole genome sequencing (digital DNA DNA Hybridization (dDDH)), 5 MLSA schemes, ferric uptake regulation (fur) and lecithin-dependent haemolysin (ldh) single gene based identification methods. Apart from dDDH which is a reference method, no technique could identify all the isolates to the species level. The other tested techniques allowed a faster, cheaper but sub genus clade identification which can be interesting when absolute precision is not required. In this regard, MALDI-ToF and fur based identification seemed especially promising.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vibrio / Vibrioses / Técnicas Bacteriológicas / Aquicultura Tipo de estudo: Diagnostic_studies / Guideline / Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Microbiol Methods Ano de publicação: 2021 Tipo de documento: Article País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vibrio / Vibrioses / Técnicas Bacteriológicas / Aquicultura Tipo de estudo: Diagnostic_studies / Guideline / Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Microbiol Methods Ano de publicação: 2021 Tipo de documento: Article País de publicação: Holanda