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High-resolution Micro-CT Myelography to Assess Spinal Subarachnoid Space Changes After Spinal Cord Injury in Rats.
Zambrano-Rodríguez, Pablo C; Bolaños-Puchet, Sirio; Reyes-Alva, Horacio J; de Los Santos, Roberto A; Martinez-Cruz, Angelina; Guízar-Sahagún, Gabriel; Medina, Luis A.
Afiliação
  • Zambrano-Rodríguez PC; Department of Neurology, Facultad de Medicina Veterinaria, Universidad Autónoma del Estado de México, Toluca, Mexico.
  • Bolaños-Puchet S; Facultad de Ciencias Veterinarias, Universidad Técnica de Manabí, Portoviejo, Ecuador.
  • Reyes-Alva HJ; Unidad de Investigación Biomédica en Cáncer INCan/UNAM, Instituto Nacional de Cancerología, Mexico City, Mexico.
  • de Los Santos RA; Department of Neurology, Facultad de Medicina Veterinaria, Universidad Autónoma del Estado de México, Toluca, Mexico.
  • Martinez-Cruz A; Unidad de Investigación Biomédica en Cáncer INCan/UNAM, Instituto Nacional de Cancerología, Mexico City, Mexico.
  • Guízar-Sahagún G; Department of Experimental Surgery, Proyecto Camina A.C., Mexico City, Mexico.
  • Medina LA; Department of Experimental Surgery, Proyecto Camina A.C., Mexico City, Mexico.
J Neuroimaging ; 31(1): 79-89, 2021 01.
Article em En | MEDLINE | ID: mdl-33244842
BACKGROUND AND PURPOSE: The spinal subarachnoid space (SSAS) is vital for neurologic function. Although SSAS alterations are known to occur after spinal cord injury (SCI), there is a lack of high-resolution imaging studies of the SSAS after SCI in rodents. Therefore, the aim here was to assess changes in the SSAS of rats subjected to graded SCI, using high-resolution micro-CT myelography. METHODS: Long-Evans adult rats were subjected to mild or severe spinal cord contusion at T9. Imaging studies of SSAS features were carried out in injured rats at acute (day 1) and subacute (day 15) stages postinjury, as well as in control rats, using high-resolution micro-CT myelography with a contrast-enhanced digital subtraction protocol. We studied a total of 33 rats randomly allocated into five experimental groups. Micro-CT myelograms were assessed by expert observers using both qualitative and quantitative criteria. RESULTS: Qualitative and quantitative analyses showed that SCI induces changes in the SSAS that vary as a function of both injury severity and time elapsed after injury. SSAS blockage was the main alteration detected. Moreover, the method used here allowed fine details to be observed in small animals, such as variations in the preferential pathways for contrast medium flow, neuroimaging nerve root enhancement, and leakage of contrast medium due to tearing of the dural sac. CONCLUSION: Micro-CT myelography provides high-resolution images of changes in the SSAS after SCI in rats and is a useful tool for further experimental studies involving rat SCI in vivo.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Medula Espinal / Traumatismos da Medula Espinal / Espaço Subaracnóideo / Mielografia / Microtomografia por Raio-X / Razão Sinal-Ruído Tipo de estudo: Qualitative_research Limite: Animals Idioma: En Revista: J Neuroimaging Assunto da revista: DIAGNOSTICO POR IMAGEM / NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Medula Espinal / Traumatismos da Medula Espinal / Espaço Subaracnóideo / Mielografia / Microtomografia por Raio-X / Razão Sinal-Ruído Tipo de estudo: Qualitative_research Limite: Animals Idioma: En Revista: J Neuroimaging Assunto da revista: DIAGNOSTICO POR IMAGEM / NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos