Epigenetic-mediated N-methyl-D-aspartate receptor changes in the brain of isolated reared rats.
Epigenomics
; 12(22): 1983-1997, 2020 11.
Article
em En
| MEDLINE
| ID: mdl-33242253
Aim: We investigated: Grin1, Grin2a, Grin2b DNA methylation; NR1 and NR2 mRNA/protein in the prefrontal cortex (PFC); and hippocampus of male Wistar rats exposed to isolation rearing. Materials & methods: Animals were kept isolated or grouped (n = 10/group) from weaning for 10 weeks. Tissues were dissected for RNA/DNA extraction and N-methyl-D-aspartate receptor subunits were analyzed using quantitative reverse transcription (RT)-PCR, ELISA and pyrosequencing. Results: Isolated-reared animals had: decreased mRNA in PFC for all markers, increased NR1 protein in hippocampus and hypermethylation of Grin1 in PFC and Grin2b in hippocampus, compared with grouped rats. Associations between mRNA/protein and DNA methylation were found for both brain areas. Conclusion: This study indicates that epigenetic DNA methylation may underlie N-methyl-D-aspartate receptor mRNA/protein expression alterations caused by isolation rearing.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Isolamento Social
/
Córtex Pré-Frontal
/
Receptores de N-Metil-D-Aspartato
/
Metilação de DNA
/
Epigênese Genética
/
Hipocampo
Aspecto:
Determinantes_sociais_saude
Limite:
Animals
Idioma:
En
Revista:
Epigenomics
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Reino Unido