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Epigenetic-mediated N-methyl-D-aspartate receptor changes in the brain of isolated reared rats.
Loureiro, Camila Marcelino; Fachim, Helene Aparecida; Corsi-Zuelli, Fabiana; Shuhama, Rosana; Joca, Sâmia; Menezes, Paulo Rossi; Dalton, Caroline F; Del-Ben, Cristina Marta; Louzada-Junior, Paulo; Reynolds, Gavin P.
Afiliação
  • Loureiro CM; Department of Internal Medicine, Division of Clinical Immunology. Ribeirão Preto Medical School, University of São Paulo, Brazil.
  • Fachim HA; Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.
  • Corsi-Zuelli F; Department of Endocrinology & Metabolism, Salford Royal Foundation Trust, Salford, UK.
  • Shuhama R; Department of Neurosciences & Behaviour, Ribeirão Preto Medical School, University of São Paulo, Brazil.
  • Joca S; Department of Neurosciences & Behaviour, Ribeirão Preto Medical School, University of São Paulo, Brazil.
  • Menezes PR; Department of Neurosciences & Behaviour, Ribeirão Preto Medical School, University of São Paulo, Brazil.
  • Dalton CF; Department of Biomolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.
  • Del-Ben CM; Aarhus Institute of Advanced Studies (AIAS), Aarhus University, Denmark.
  • Louzada-Junior P; Department of Preventive Medicine, Faculty of Medicine, University of São Paulo, Brazil.
  • Reynolds GP; Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.
Epigenomics ; 12(22): 1983-1997, 2020 11.
Article em En | MEDLINE | ID: mdl-33242253
Aim: We investigated: Grin1, Grin2a, Grin2b DNA methylation; NR1 and NR2 mRNA/protein in the prefrontal cortex (PFC); and hippocampus of male Wistar rats exposed to isolation rearing. Materials & methods: Animals were kept isolated or grouped (n = 10/group) from weaning for 10 weeks. Tissues were dissected for RNA/DNA extraction and N-methyl-D-aspartate receptor subunits were analyzed using quantitative reverse transcription (RT)-PCR, ELISA and pyrosequencing. Results: Isolated-reared animals had: decreased mRNA in PFC for all markers, increased NR1 protein in hippocampus and hypermethylation of Grin1 in PFC and Grin2b in hippocampus, compared with grouped rats. Associations between mRNA/protein and DNA methylation were found for both brain areas. Conclusion: This study indicates that epigenetic DNA methylation may underlie N-methyl-D-aspartate receptor mRNA/protein expression alterations caused by isolation rearing.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Isolamento Social / Córtex Pré-Frontal / Receptores de N-Metil-D-Aspartato / Metilação de DNA / Epigênese Genética / Hipocampo Aspecto: Determinantes_sociais_saude Limite: Animals Idioma: En Revista: Epigenomics Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Isolamento Social / Córtex Pré-Frontal / Receptores de N-Metil-D-Aspartato / Metilação de DNA / Epigênese Genética / Hipocampo Aspecto: Determinantes_sociais_saude Limite: Animals Idioma: En Revista: Epigenomics Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido