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Improvement of endothelial function by Gunnera tinctoria extract with antioxidant properties.
Sabando, Constanza; Rodríguez-Díaz, Maité; Ide, Walther; Pastene, Edgar; Avello, Marcia; Simirgiotis, Mario; Rojas, Susana; Villarroel, Enrique; Silva-Grecchi, Tiare; Gutiérrez, Cristian; Bouza, Rebeca; Cicchelli, Bárbara; González, Marcelo; Rodríguez-Llamazares, Saddys.
Afiliação
  • Sabando C; Centro de Investigación de Polímeros Avanzados (CIPA), Avda. Collao 1202, Edificio de Laboratorios, Concepción, Chile.
  • Rodríguez-Díaz M; Facultad de Medicina, Universidad Andrés Bello, República 590, Santiago, Chile.
  • Ide W; Centro de Investigación de Polímeros Avanzados (CIPA), Avda. Collao 1202, Edificio de Laboratorios, Concepción, Chile.
  • Pastene E; Laboratorio de Farmacognosia, Departamento de Farmacia, Facultad de Farmacia, Universidad de Concepción, Concepción, Chile.
  • Avello M; Laboratorio de Síntesis y Biotransformación de Productos Naturales, Universidad del Bío-Bío, Avda. Andrés Bello 720, Chillán, Chile.
  • Simirgiotis M; Laboratorio de Farmacognosia, Departamento de Farmacia, Facultad de Farmacia, Universidad de Concepción, Concepción, Chile.
  • Rojas S; Instituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile.
  • Villarroel E; Laboratorio de Fisiología Vascular, Departamento de Fisiología, Facultad de Ciencias Biológicas, Universidad de Concepción, P.O. Box 160-C, Concepción, Chile.
  • Silva-Grecchi T; Laboratorio de Fisiología Vascular, Departamento de Fisiología, Facultad de Ciencias Biológicas, Universidad de Concepción, P.O. Box 160-C, Concepción, Chile.
  • Gutiérrez C; Laboratorio de Screening de Compuestos Neuroactivos, Departamento de Fisiología, Facultad de Ciencias Biológicas, Universidad de Concepción, P.O. Box 160-C, Concepción, Chile.
  • Bouza R; Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, P.O. Box 160-C, Concepción, Chile.
  • Cicchelli B; Departamento de Física, E.U.P. Ferrol, Universidad de A Coruña, Avda. 19 de Febrero, s/n, 15405, Ferrol, Spain.
  • González M; Laboratorio de Investigación Materno-Fetal (LIMaF), Departamento de Obstetricia y Ginecología, Facultad de Medicina, Universidad de Concepción, P.O. Box 160-C, Concepción, Chile.
  • Rodríguez-Llamazares S; Laboratorio de Fisiología Vascular, Departamento de Fisiología, Facultad de Ciencias Biológicas, Universidad de Concepción, P.O. Box 160-C, Concepción, Chile. mgonzalezo@udec.cl.
Biol Res ; 53(1): 55, 2020 Nov 23.
Article em En | MEDLINE | ID: mdl-33228801
BACKGROUND: Gunnera tinctoria has been collected by Mapuche-Pewenche people for food and medicinal purposes. The high polyphenol content of methanolic extract from G. tinctoria leaves with chemical constituents such as ellagic acid and quercetin derivatives suggests its application to prevent endothelial dysfunction and oxidative stress. The aim of this study was to provide evidence of the protective effect of this extract on endothelial function by reducing oxidative stress induced by high D-glucose and H2O2, as well as by stimulating nitric oxide (NO) levels in human umbilical vein endothelial cells (HUVECs). RESULTS: A methanolic extract with a high content of polyphenols (520 ± 30 mg gallic acid equivalents/g dry extract) was obtained from G. tinctoria leaves. Its main constituent was ellagic acid. The results of Ferric reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays of the extract confirmed its antioxidant activity by inhibition pathway of radical species. The incubation of HUVECs with the extract decreased the apoptosis and reactive oxygen species (ROS) synthesis induced by high extracellular concentration of D-glucose or hydrogen peroxide. The extract increased endothelial NO levels and reduced vasoconstriction in human placental vessels. CONCLUSIONS: This study provides evidence about the antioxidant and endothelial protective properties of methanolic G. tinctoria leaf extract. The extract improves the availability of NO in HUVECs, inhibiting the production of ROS and vasoconstriction.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Estresse Oxidativo / Células Endoteliais da Veia Umbilical Humana / Peróxido de Hidrogênio / Antioxidantes Limite: Female / Humans / Pregnancy Idioma: En Revista: Biol Res Assunto da revista: BIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Estresse Oxidativo / Células Endoteliais da Veia Umbilical Humana / Peróxido de Hidrogênio / Antioxidantes Limite: Female / Humans / Pregnancy Idioma: En Revista: Biol Res Assunto da revista: BIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido