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Expression and DNA methylation profiles of EZH2-target genes in plasma exosomes and matched primary tumor tissues of the patients with diffuse large B-cell lymphoma.
Baris, I C; Hacioglu, S; Turk, N S; Cetin, G O; Zencir, S; Bagci, G; Caner, V.
Afiliação
  • Baris IC; Department of Medical Biology, School of Medicine, Pamukkale University, Denizli, Turkey.
  • Hacioglu S; Department of Hematology, School of Medicine, Pamukkale University, Denizli, Turkey.
  • Turk NS; Department of Medical Pathology, School of Medicine, Pamukkale University, Denizli, Turkey.
  • Cetin GO; Department of Medical Genetics, School of Medicine, Pamukkale University, Denizli, Turkey.
  • Zencir S; Department of Medical Biology, School of Medicine, Pamukkale University, Denizli, Turkey.
  • Bagci G; Department of Molecular Biology, University of Geneva, 1211, Geneva 4, Switzerland.
  • Caner V; Department of Medical Genetics, School of Medicine, Pamukkale University, Denizli, Turkey.
Clin Transl Oncol ; 23(6): 1152-1166, 2021 Jun.
Article em En | MEDLINE | ID: mdl-33226554
AIMS: Diffuse large B-cell lymphoma (DLBCL) is the most common type of aggressive lymphoma. This study was designed to compare epigenetic alterations observed in Enhancer of Zeste Homolog 2 (EZH2)-target genes between plasma-derived exosomes and primary tumors in DLBCL patients. MAIN METHODS: Exosomes were isolated from plasma of 21 DLBCL patients and 21 controls. We analyzed the methylation status of the target genes using methylation-specific PCR. We also examined whether the exosomes and the tumor samples contained transcripts of the target genes. KEY FINDINGS: We found that CDKN2A and CDKN2B were methylated in both plasma exosomes and primary tumor tissue samples. None of the transcripts were found in the exosomes except CDKN1B which was expressed in 8 (38%) of the exosome samples. SIGNIFICANCE: This study showed that plasma exosomes might preferably package certain target molecules from primary tumors and the exosomes containing dual methylated DNAs of CDKN2A and CDKN2B, or CDKN1B transcript may contribute to DLBCL pathogenesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação Neoplásica da Expressão Gênica / Linfoma Difuso de Grandes Células B / Metilação de DNA / Exossomos / Proteína Potenciadora do Homólogo 2 de Zeste Limite: Humans Idioma: En Revista: Clin Transl Oncol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Turquia País de publicação: Itália
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação Neoplásica da Expressão Gênica / Linfoma Difuso de Grandes Células B / Metilação de DNA / Exossomos / Proteína Potenciadora do Homólogo 2 de Zeste Limite: Humans Idioma: En Revista: Clin Transl Oncol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Turquia País de publicação: Itália