A green, simplified, and efficient experimental setup for a high-throughput screening of agri-food by-products - From polar to nonpolar metabolites in sugarcane solid residues.
J Chromatogr A
; 1634: 461693, 2020 Dec 20.
Article
em En
| MEDLINE
| ID: mdl-33220587
From an environmental perspective, searching for useful compounds in agri-food by-products by employing inefficient and polluting analytical procedures is paradoxical. This work aimed to develop a green, simplified, and highly efficient experimental setup for extracting and tentatively identifying the broadest range of metabolites in sugarcane solid by-products collected directly within the industrial mills. Nine different extraction approaches were investigated side-by-side, including three reference methods. Based on the extraction and environmental performances assessed by two complementary metrics called Analytical-Eco Scale and the Analytical Greenness Calculator, it was possible to reach two highly efficient two liquid-phase extractions while avoiding harmful solvents and traditional time, energy, and solvent consuming sample preparation steps, such as solvent evaporation, metabolite concentration, re-suspension, and derivatization. The simultaneously produced hydroethanolic and n-heptane extracts were directly analyzed by ultra-high-performance liquid chromatography and gas chromatography, both coupled to mass spectrometry, respectively, leading to the annotation of a large dynamic range of compounds from information rich spectral data. Up to 111 metabolites were identified in a single matrix, from highly polar sucrose to nonpolar wax ester C53 in a single extraction. Orientin, apigenin-6-C-glucosylrhamnoside, 1-octacosanol, octacosanal, and other bioactive compounds were identified in these abundantly available by-products, which are currently just burned to produce energy. The best two methods developed here (Two-Liquid-Phase Ultrasound-Assisted Extraction with Probe and Two-Liquid-Phase Dynamic Maceration) appeared as a green, simplified, and highly efficient procedures to qualitatively profile metabolites in complex solid matrices.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharum
/
Poluentes Ambientais
/
Química Verde
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Ensaios de Triagem em Larga Escala
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Análise de Alimentos
Tipo de estudo:
Diagnostic_studies
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Screening_studies
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Holanda