Micromonospora fluminis sp. nov., isolated from mountain river sediment.
Int J Syst Evol Microbiol
; 70(12): 6428-6436, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-33174828
During a bioprospection of bacteria with antimicrobial activity, the actinomycete strain A38T was isolated from a sediment sample of the Carpintero river located in the Gran Piedra Mountains, Santiago de Cuba province (Cuba). This strain was identified as a member of the genus Micromonospora by means of a polyphasic taxonomy study. Strain A38T was an aerobic Gram-positive filamentous bacterium that produced single spores in a well-developed vegetative mycelium. An aerial mycelium was absent. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, mannose, ribose and xylose. The major cellular fatty acids were isoC15:0, 10 methyl C17:0, anteiso-C17:0 and iso-C17:0. The predominant menaquinones were MK-10(H4) and MK-10(H6). Phylogenetic analysis of 16S rRNA gene sequences revealed that this strain was closely related to Micromonospora tulbaghiae DSM 45142T (99.5â%), Micromonospora citrea DSM 43903T (99.4â%), Micromonospora marina DSM 45555T (99.4â%), Micromonospora maritima DSM 45782T (99.3â%), Micromonospora sediminicola DSM 45794T (99.3â%), Micromonospora aurantiaca DSM 43813T (99.2â%) and Micromonospora chaiyaphumensis DSM 45246T (99.2â%). The results of OrthoANIu analysis showed the highest similarity to Micromonospora chalcea DSM 43026T (96.4â%). However, the 16S rRNA and gyrB gene sequence-based phylogeny and phenotypic characteristics provided support to distinguish strain A38T as a novel species. On the basis of the results presented here, we propose to classify strain A38T (=LMG 30467T=CECT 30034T) as the type strain of the novel species Micromonospora fluminis sp. nov.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Sedimentos Geológicos
/
Rios
/
Micromonospora
Tipo de estudo:
Prognostic_studies
País/Região como assunto:
Caribe
/
Cuba
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Bélgica
País de publicação:
Reino Unido