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Nitric Oxide Synthase inhibition counteracts the stress-induced DNA methyltransferase 3b expression in the hippocampus of rats.
de Sousa Maciel, Izaque; Sales, Amanda J; Casarotto, Plinio C; Castrén, Eero; Biojone, Caroline; Joca, Sâmia R L.
Afiliação
  • de Sousa Maciel I; School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto - SP, Brazil.
  • Sales AJ; School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto - SP, Brazil.
  • Casarotto PC; Neuroscience Center, HiLIFE, University of Helsinki, Finland.
  • Castrén E; Neuroscience Center, HiLIFE, University of Helsinki, Finland.
  • Biojone C; Neuroscience Center, HiLIFE, University of Helsinki, Finland.
  • Joca SRL; Department of Biomolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto -SP, Brazil.
Eur J Neurosci ; 55(9-10): 2421-2434, 2022 05.
Article em En | MEDLINE | ID: mdl-33170977
It has been postulated that the activation of NMDA receptors (NMDAr) and nitric oxide (NO) production in the hippocampus is involved in the behavioral consequences of stress. Stress triggers NMDAr-induced calcium influx in limbic areas, such as the hippocampus, which in turn activates neuronal NO synthase (nNOS). Inhibition of nNOS or NMDAr activity can prevent stress-induced effects in animal models, but the molecular mechanisms behind this effect are still unclear. In this study, cultured hippocampal neurons treated with NMDA or dexamethasone showed an increased of DNA methyltransferase 3b (DNMT3b) mRNA expression, which was blocked by pre-treatment with nNOS inhibitor nω -propyl-l-arginine (NPA). In rats submitted to the Learned Helplessness paradigm (LH), we observed that inescapable stress increased DNMT3b mRNA expression at 1h and 24h in the hippocampus. The NOS inhibitors 7-NI and aminoguanidine (AMG) decreased the number of escape failures in LH and counteracted the changes in hippocampal DNMT3b mRNA induced in this behavioral paradigm. Altogether, our data suggest that NO produced in response to NMDAr activation following stress upregulates DNMT3b in the hippocampus.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxido Nítrico Sintase / Hipocampo Limite: Animals Idioma: En Revista: Eur J Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: França
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxido Nítrico Sintase / Hipocampo Limite: Animals Idioma: En Revista: Eur J Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: França